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ProteomicsProximity proteomics of phagophore-associated proteins using APEX2-LC3B in VPS37A KO U-2 OS cells
We utilized an APEX2-mediated proximity biotinylation approach to label proteins on LC3-positive membranes that accumulate in VPS37A-knockout (KO) U-2 OS cells. AP, APEX2 control; WT, APEX2-LC3B.
ORGANISM(S): Homo sapiens (Human) 
2024-12-03 | PXD057301 | Pride
Human Vps37a ko u-2 os cells Autophagosomal membrane Phagophore Apex2-lc3b 
TranscriptomicsEndosome-associated lc3b mRNA is translated in axons and the protein contributes to endosomal clearance
The primary objective of this project was to identify transcripts in close proximity to endosomes using the APEX-seq approach. To achieve this, we employed SH-SY5Y cells expressing APEX2-NES, APEX2-RAB5A, or 2xFYVE-APEX2 constructs. Biotinylation of RNAs was validated by dot blot, and biotinylated R...
ORGANISM(S): Mus musculus 
2026-09-01 | E-MTAB-15783 | biostudies-arrayexpress
GenomicsEndosome-associated lc3b mRNA is translated in axons and the protein contributes to endosomal clearance
Endosome-associated lc3b mRNA is translated in axons and the protein contributes to endosomal clearance
PRJEB101068 | ENA
ProteomicsFinding the LC3-interactors in senescent human A549 cells during influenza A virus infection
To screen age-related regulators of mitophagy, we examined LC3-interacting proteins by proximity-dependent biotin identification. LC3B was cloned into a mammalian expression vector tagged with a mitochondrial OMM-targeted APEX2 (mito-APEX) label for proximity-based identification of LC3-interacting ...
ORGANISM(S): Homo sapiens (Human) 
2025-09-29 | PXD057110 | Pride
Influenza a virus Lc3 Ifection Senescent human cells 
GenomicsTranslation in Proximity to Forming Autophagosomes During Sustained Autophagy
Translation in Proximity to Forming Autophagosomes During Sustained Autophagy
PRJNA1423328 | ENA
ProteomicsDNA Damage in Macrophages Drives Immune Autoreactivity via Nuclear Antigen Presentation
Aging and DNA damage increase the risk of chronic inflammation and autoimmunity, yet their molecular underpinnings remain unclear. Here, we uncover a DNA damage-driven mechanism in macrophages that triggers immune autoreactivity. Using Er1Lyz2/- animals with a macrophage-specific DNA repair defect i...
ORGANISM(S): Homo sapiens (Human) 
2025-12-03 | PXD058936 | Pride
Macrophages Genome stability Autophagy Immunosenescence Autophagosome profiling 
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