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ProteomicsSeb1 proximity dependent biotinylation (PDB) in Schizosaccharomyces pombe
Transcription by RNA polymerase I (RNAPI) represents most of the transcriptional activity in eukaryotic cells and is associated with the production of mature ribosomal RNA (rRNA). As several rRNA maturation steps are coupled to RNAPI transcription, the rate of RNAPI elongation directly influences pr...
ORGANISM(S): Schizosaccharomyces pombe 
2023-05-02 | PXD037768 | Pride
Schizosaccharomyces pombe Seb1 Turboid 
UnknownHow Funding Policy Maintains Structural Inequity Within Indigenous Community-Based Organizations.
Not available
S-EPMC12462777 | biostudies-literature
ProteomicsProximity-dependent biotinylation by TurboID to identify protein-protein interaction networks in yeast
The use of proximity-dependent biotinylation assays coupled to mass spectrometry (PDB-MS) has changed the field of protein-protein interactions (PPI) studies. Yet, despite the recurrent and successful use of BioID-based PPI screening in mammalian cells, the implementation of PDB-MS in yeast has not ...
ORGANISM(S): Schizosaccharomyces pombe 927 
2019-05-16 | PXD013183 | Pride
Proximity-dependent biotinylation  turboid  yeast  s. pombe  rmt3 Rna exosome 
ProteomicsGlobal profiling and functional characterization of post-translational modifications of the fission yeast RNA exosome
The RNA exosome is a multi-subunit complex essential for processing and degradation of many classes of RNA. Although many of the functions of the RNA exosome are well established, how the activity of this complex is regulated remains poorly understood. Here we report a comprehensive proteomic analys...
ORGANISM(S): Schizosaccharomyces pombe 927 
2019-01-21 | PXD009529 | Pride
Fission yeast Post-translational modification Rna exosome Gene expression regulation 
TranscriptomicsThe Nrd1-like protein Seb1 coordinates co-transcriptional 3’ end processing and polyadenylation site selection
Termination of RNAPII transcription is associated with RNA 3’ end formation. For coding genes, termination is initiated by the cleavage/polyadenylation machinery. In contrast, a majority of noncoding transcription events in S. cerevisiae do not rely on RNA cleavage for termination, but instead ter...
ORGANISM(S): Schizosaccharomyces pombe 
2016-07-22 | E-GEOD-82326 | biostudies-arrayexpress
ProteomicsRepression of pervasive transcription is the primary role of RNA polymerase II CTD serine 2 phosphorylation in fission yeast
The RNA polymerase II carboxy-terminal domain (CTD) consists of conserved repeats of the consensus sequence Y1-S2-P3-T4-S5-P6-S7, which can be phosphorylated to influence distinct stages of the transcription cycle, including RNA processing. Although affinity purification coupled with mass spectromet...
ORGANISM(S): Schizosaccharomyces pombe Schizosaccharomyces pombe strain SPY73 975 h+ 
2024-05-06 | PXD047023 | Pride
Turboid Set2 Antiisense repression Ctd Rna polymerase ii 
UnknownPRMT3 is a ribosomal protein methyltransferase that affects the cellular levels of ribosomal subunits.
Not available
S-EPMC449775 | biostudies-literature
UnknownProteomic profiling and functional characterization of post-translational modifications of the fission yeast RNA exosome.
Not available
S-EPMC6265454 | biostudies-literature
UnknownNuclear export competence of pre-40S subunits in fission yeast requires the ribosomal protein Rps2.
Not available
S-EPMC2577348 | biostudies-literature
UnknownThe THO complex cooperates with the nuclear RNA surveillance machinery to control small nucleolar RNA expression.
Not available
S-EPMC3488260 | biostudies-literature
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