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ProteomicsSimple and Fast Maximally Deuterated Control (maxD) preparation for HDX MS Experiments
During the analysis steps of hydrogen deuterium exchange (HDX) mass spectrometry (MS) there is an unavoidable loss of deuterons, or back-exchange. Understanding back-exchange is necessary to correct for loss during analysis, to calculate the absolute amount of exchange, and to ensure that deuterium ...
ORGANISM(S): Bos taurus (Bovine) Homo sapiens (Human) Equus caballus (Horse) 
2022-07-08 | PXD032924 | Pride
Hdx ms Mass spectrometry Hydrogen deuterium exchange Back-exchange Maximally deuterated samples 
UnknownMinimizing back exchange in the hydrogen exchange-mass spectrometry experiment.
Not available
S-EPMC3515739 | biostudies-literature
UnknownChromatography at -30 °C for Reduced Back-Exchange, Reduced Carryover, and Improved Dynamic Range for Hydrogen-Deuterium Exchange Mass Spectrometry.
Not available
S-EPMC9264389 | biostudies-literature
ProteomicsRole of Complementarity-Determining Regions 1 and 3 in Pathologic Amyloid Formation by Human Immunoglobulin κ1 Light Chains
Immunoglobulin light chain (LC) amyloidosis is a life-threatening disease whose understanding and treatment is complicated by vast numbers of patient-specific mutations. To address molecular origins of the disease, we explored 14 patient-derived and engineered proteins related to κ1-family germline ...
ORGANISM(S): Homo sapiens (Human) 
2023-05-23 | PXD039682 | Pride
Light chain amyloidosis  misfolded states  protein stability  protein dynamics  hdx ms  hydrogen deuterium exchange mass spectrometry 
UnknownProtein deuteration <i>via</i> algal amino acids to circumvent proton back-exchange for <sup>1</sup>H-detected solid-state NMR.
Not available
S-EPMC10928984 | biostudies-literature
UnknownMinimizing back exchange in 18O/16O quantitative proteomics experiments by incorporation of immobilized trypsin into the initial digestion step.
Not available
S-EPMC2796076 | biostudies-literature
ProteomicsStructural mechanism underlying primary and secondary coupling between GPCRs and the Gi/o family
G protein-coupled receptors (GPCRs) are the largest receptor superfamily that can propagate various extracellular stimulus into cells by coupling with heterotrimeric G proteins. G proteins are divided into four families, Gs, Gi/o, Gq/11 and G12/13, which are responsible for the transduction of discr...
ORGANISM(S): Homo sapiens (Human) 
2020-07-02 | PXD019367 | Pride
Primary couplling Secondary coupling Gpcr G protein Hdx-ms 
ProteomicsLigand-induced shifts in conformational ensembles that predict transcriptional activation
Nuclear receptors function as ligand-regulated transcription factors whose ability to regulate diverse physiological processes is closely linked with conformational changes induced upon ligand binding. Understanding how conformational populations of nuclear receptors are shifted by various ligands c...
ORGANISM(S): Homo sapiens (Human) 
2022-10-12 | PXD036076 | Pride
Lc-msms 
ProteomicsStructural Premise of Selective Deubiquitinase USP30 Inhibition by Small-Molecule Benzosulfonamides
Dampening functional levels of the mitochondrial deubiquitylating enzyme USP30 has been suggested as an effective therapeutic strategy against neurodegenerative disorders such as Parkinson’s Disease. USP30 inhibition may counteract the deleterious effects of impaired turnover of damaged mitochondria...
ORGANISM(S): Homo sapiens (Human) 
2023-10-24 | PXD041582 | Pride
Abpp-ms Usp30 Hdx-ms 
ProteomicsMolecular mechanism of muscarinic acetylcholine receptor M3 interaction with Gq
Muscarinic acetylcholine receptor M3 (M3) and its downstream effector Gq/11 are critical drug development targets given their involvement in numerous physiological processes and diseases. Although a cryo-electron microscopy study previously defined the structure of the M3-miniGq complex, the lack of...
ORGANISM(S): Homo sapiens (Human) 
2024-05-22 | PXD042562 | Pride
Hdx-ms M3 G protein Gq Gpcr 
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