Our method (Ub-POD) exploits the proximity and the relative orientation of the E3-ligase catalytic domain with respect to ubiquitin observed in the enzymatic intermediate-state structures of E3-E2~Ub. By fusing biotin ligase BirA and an Avi tag variant to the candidate E3 ligase and ubiquitin, respe...
ORGANISM(S): Homo sapiens (Human)