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ProteomicsMetaproteome of the waste water treatment plant Gerwisch/Germany
In this study the impact of protein fractionation techniques prior to LC/MS analysis was investigated on activated sludge samples derived at winter and summer condition from a full-scale wastewater treatment plant (WWTP). For reduction of the sample complexity, different fractionation techniques inc...
ORGANISM(S): Bacteria 
2015-07-28 | PXD001547 | Pride
Metaproteomics Activated sludge Metaproteomeanalyzer Wastewater treatment plant Offgel isoelectric focusing 
ProteomicsMetaproteomic analysis of a mixed microbial communities extracted from biogas plant sludge
In this study, centrifugal fractionation has been applied for fractionation of two biogas sludge samples to analyze proteins extracted from (i) crude fibers, (ii) suspended microorganisms, and (iii) secreted proteins in the supernatant using a gel-based approach followed by LC-MS/MS identification.
ORGANISM(S): Zea mays (Maize) 
2015-07-21 | PXD001508 | Pride
Metabolism Microbial communities Metaproteomics Differential centrifugation Biogas plant 
ProteomicsFecal metaproteomics reveals reduced gut inflammation and changed microbial metabolism following lifestyle-induced weight loss
Aims Gut microbiota-mediated inflammation promotes obesity-associated low-grade inflammation, which represents a hallmark of the metabolic syndrome (MetS). Lifestyle-induced weight loss (WL) is regarded as an efficient therapy to reverse MetS and to prevent disease progression. The objective of this...
ORGANISM(S): human gut metagenome 
2021-04-19 | PXD020902 | Pride
Metaproteomics Obesity Metabolic syndrome Microbiome Gut inflammation Weight loss 
ProteomicsQuantitative analysis of proteomic changes in monoclonal MDCK cell lines infected with human influenza A virus
Proteomics of Influenza A virus infected MDCK cell clones. Spike-in of AQUA peptide for five major influenza A virus proteins plus measurement of the host cell proteome and comparison of changes for the two cell clones.
ORGANISM(S): Canis familiaris (Dog) (Canis lupus familiaris) 
2025-09-29 | PXD067996 | Pride
-quantitative mass spectrometry -host cell proteins -influenza a virus -mdck cell lines -dia-pasef 
ProteomicsAdaptation of a microbial community to demand-oriented biological methanation
Background: Biological conversion of the surplus of renewable electricity to CH4 could support energy storage and strengthen the power grid. Biological methanation (BM) is closely linked to the activity of biogas-producing bacterial community and methanogenic Archaea in particular. During reactor o...
ORGANISM(S): Escherichia coli 
2023-03-11 | PXD034618 | Pride
Biogas upgrade Biological methanation Hydrogen starvation Renewable energy Hydrogenotrophic methanogens Acetoclastic methanogens Power to methane Microbial food-web Metaproteomics 
ProteomicsDetection, isolation and characterization of phage-host complexes using BONCAT and click chemistry
Phages are viruses that infect prokaryotes and can shape microbial communitiesby lysis, thus offering applications in various fields. However, challengesexist in sampling, isolation and accurate prediction of the host specificity ofphages as well as in the identification of newly replicated virions ...
ORGANISM(S): Escherichia phage Lambda 
2024-08-29 | PXD044316 | Pride
Phage Fluorescence Proteomics Boncat labeling Host range Sequencing Click chemistry Biotin Lc-ms/ms 
ProteomicsGiant panda (Ailuropoda melanoleuca) gut microbiomes degrade bamboo in distinct patterns: heterofermentation of green leaves to ethanol, lactate and acetate, and homofermentation of yellow pith to lactate
The iconic giant panda is an endangered species known worldwide for its peculiar dietary habits. While retaining the digestive system of a carnivore, the giant panda successfully moved into a diet almost exclusively based on bamboo. Digestion of lignocellulose is believed to be conducted solely by i...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) Escherichia coli 
2020-03-02 | PXD010872 | Pride
Metaproteomics microbial communities fermentation 
ProteomicsMetaproteomics of fecal samples supports the diagnosis of Crohn’s Disease and Ulcerative Colitis
Objective Crohn’s Disease (CD) and Ulcerative Colitis (UC) are chronic inflammatory diseases of the gastrointestinal tract. Reliable diagnosis of these diseases requires a comprehensive examination of the patient, which include invasive endoscopy. This study assesses whether non-invasive LC-MS/MS ba...
ORGANISM(S): Homo sapiens (Human) 
2019-03-11 | PXD010371 | Pride
• fecal samples • metaproteomics • non-invasive diagnosis • inflammatory bowel disease • crohn’s disease • ulcerative colitis • gastrointestinal tract 
ProteomicsNo evidence for major spacial differences within the microbial communities in anaerobic digestion plants under different feeding and stirring regimes
In anaerobic digestion plants (ADP) homogenization of the feed, the fermenter content and the microbial communities represents a precondition for effective and robust biogas production,but also a major energy consumer. For a 850 m3 agricultural AD-P equipped with eight sampling ports we investigated...
ORGANISM(S): Escherichia coli 
2021-04-27 | PXD016213 | Pride
Microbial communities Stirring Trflp Anaerobic digestion  biogas Metaproteomics 
ProteomicsProteotyping of microbial communities reveals the correlations between the microorganisms and the process parameters
Background: Methane yield and biogas productivity of biogas plants depend on microbial community structure and functionality, substrate supply, and general process parameters. Little is known, however, about the correlations between microbial community function and the process parameters. To close t...
ORGANISM(S): Acyrthosiphon pisum (Pea aphid) 
2016-04-19 | PXD003526 | Pride
Biogas plant Biogas Anaerobic digestion Complex microbial community 
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