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ProteomicsSuper-resolution proximity labeling method with enhanced direct identification of biotinylation sites
Promiscuous labeling enzymes, such as APEX2 or TurboID, are commonly used in in situ biotinylation studies of subcellular proteomes or protein-protein interactions. Although the conventional approach of enriching biotinylated proteins is widely implemented, in-depth identification of specific biotin...
ORGANISM(S): Homo sapiens (Human) 
2024-05-22 | PXD047979 | Pride
Biotinylation site Apex2 Turboid Lc-ms Membrane protein topology Bioid Proximity labeling 
ProteomicsThiol-cleavable Biotin for Chemical and Enzymatic Biotinylation and its Application to Mitochondrial TurboID Proteomics
Protein biotinylation via chemical or enzymatic reactions is often coupled with streptavidin-based enrichment and on-beads digestion in numerous biological applications. However, the popular on-beads digestion method faces major challenges of streptavidin contamination, the lost information of bioti...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2021-04-21 | MSV000087256 | MassIVE
UnknownSuper-resolution proximity labeling with enhanced direct identification of biotinylation sites.
Not available
S-EPMC11082246 | biostudies-literature
UnknownAntibodies to biotin enable large-scale detection of biotinylation sites on proteins.
Not available
S-EPMC8954634 | biostudies-literature
ProteomicsBIOSITe: A novel method for direct detection of site specific biotinylation
Proximity-dependent biotinylation methods, such as those mediated by BioID and APEX, are being widely adopted for studying in vivo protein-protein interaction dynamics and subcellular structures. However, these along with other biotin-based technologies are severely dependent on avidin family protei...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2017-12-19 | PXD007862 | Pride
Bioid Proximity dependent biotinylation Bcr abl Mass spectrometry Ip Peptide Biotinylation Biosite 
UnknownLocalized protein biotinylation at DNA damage sites identifies ZPET, a repressor of homologous recombination.
Not available
S-EPMC6317314 | biostudies-literature
UnknownA comprehensive LC-MS based study of factors influencing biotinylation of critical reagents.
Not available
S-EPMC11457598 | biostudies-literature
ProteomicsAntibodies to biotin enable large-scale detection of biotinylation sites on proteins
Udeshi ND, Pedram K, Svinkina T, Fereshetian S, Myers SA, Aygun O, Krug K, Clauser KR, Ryan D, Ast T, Mootha VK, Ting AY, Carr SA. Nature Methods 2017. Although purification of biotinylated molecules is highly efficient, identifying specific sites of biotinylation remains challenging. We show that a...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2017-08-31 | MSV000081496 | MassIVE
UnknownThiol-Cleavable Biotin for Chemical and Enzymatic Biotinylation and Its Application to Mitochondrial TurboID Proteomics.
Not available
S-EPMC8898397 | biostudies-literature
UnknownBioSITe: A Method for Direct Detection and Quantitation of Site-Specific Biotinylation.
Not available
S-EPMC6092923 | biostudies-literature
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