Sort   by:  
 Page size 
ProteomicsAP-4 vesicles contribute to spatial control of autophagy via RUSC-dependent peripheral delivery of ATG9A
Adaptor protein 4 (AP-4) is an ancient membrane trafficking complex, whose function has largely remained elusive. In humans, AP-4 deficiency causes a severe neurological disorder of unknown aetiology. We apply unbiased proteomic methods, including ‘Dynamic Organellar Maps’, to find proteins whose su...
ORGANISM(S): Homo sapiens (Human) 
2018-09-27 | PXD010103 | Pride
Human cells Autophagy Dynamic organellar maps Ap-4 Vesicles 
ProteomicsHigh-Content Screening Identifies A Small Molecule That Restores AP-4-dependent Protein Trafficking In Neuronal Models Of AP-4-Associated Hereditary Spastic Paraplegia
Unbiased phenotypic screens in patient-relevant disease models offer the potential to detect therapeutic targets for rare diseases. In this study, we developed a high-throughput screening assay to identify molecules that correct aberrant protein trafficking in adaptor protein complex 4 (AP-4) defici...
ORGANISM(S): Homo sapiens (Human) 
2024-01-26 | PXD042950 | Pride
Human Autophagy Ipsc Drug screen Protein trafficking Hsp 
ProteomicsA regulatory cascade reprograms the ubiquitin ligase Ubr1 to enhance protein quality control during stress
When faced with proteotoxic stress, cells mount adaptive responses to eliminate aberrant proteins. Adaptive responses enhance the cellular quality control machinery by increasing expression of protein folding and degradation factors, but it is unclear if and how this augmented machinery acquires new...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2018-06-06 | PXD008962 | Pride
Lc-msms Yeast Qexactive hf Orbitrap 
ProteomicsJurkat extracellular vesicle fractionation profiling
Extracellular vesicles are a mixture of particles of variable size and subcellular origin. To characterize the different vesicle species further, we applied differential centrifugation and profiling to Jurkat cell conditioned medium. The three step centriguation gradient yielded sufficient partial s...
ORGANISM(S): Homo sapiens (Human) 
2021-02-26 | PXD019074 | Pride
Extracellular vesicles Profiling Silac Fractionation Jurkat Differential centrifugation 
Sort   by:  
 Page size