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ProteomicsAn Amphipathic Helix in Brl1 is Required for Nuclear Pore Complex Biogenesis in S. cerevisiae - KARMA analysis of Brl1 (DIA)
This project was aimed at analyzing the binding of Brl1 to the nuclear pore complex during the assembly process using the Kinetic Analysis of incorporation Rates in Macromolecular Assemblies (KARMA, Onischenko et al. 2020) method. In brief, we affinity purified Brl1 at several time points following ...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2022-08-17 | PXD032017 | Pride
Dia Karma 
ProteomicsAn Amphipathic Helix in Brl1 is Required for Nuclear Pore Complex Biogenesis in S. cerevisiae - KARMA analysis of Nup170 with/without Brl1 degradation (DIA)
This project was aimed at investigating nuclear pore complex biogenesis upon inducible degradation of Brl1 using the Kinetic Analysis of incorporation Rates in Macromolecular Assemblies (KARMA, Onischenko et al. 2020) method. In brief, the nuclear pore complex was purified through endogenously tagge...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2022-08-17 | PXD032024 | Pride
Dia Karma 
ProteomicsAn Amphipathic Helix in Brl1 is Required for Nuclear Pore Complex Biogenesis in S. cerevisiae – targeted analysis of cell lysate (PRM)
The project was aimed at assessing the metabolic labeling of NUPs in the full lysate upon SILAC labeling of growing yeast cultures, 90 min post labeling. This experiment was performed to exclude that the systematic labeling differences that we observed in KARMA assays with Brl1 bait were present alr...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2022-08-17 | PXD032016 | Pride
Cell lysate Prm 
ProteomicsAn Amphipathic Helix in Brl1 is Required for Nuclear Pore Complex Biogenesis in S. cerevisiae - Lysate intermixing text (DIA)
The project was aimed at assessing the dynamic protein exchange of the nuclear pore complex during the affinity purification procedure with Brl1 and Nup170 bait. In brief, equal fractions of an affinity tagged strain grown in unlabeled medium and a wild-type culture grown in metabolically labeled me...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2022-08-17 | PXD032034 | Pride
Dia 
ProteomicsThe histone acetyltransferase Mst2 protects active chromatin from epigenetic silencing by acetylating the ubiquitin ligase Brl1
Faithful propagation of functionally distinct chromatin states is crucial for maintaining cellular identity, and its breakdown can lead to diseases such as cancer. Whereas mechanisms that sustain repressed states have been intensely studied, regulatory circuits that protect active chromatin from ina...
ORGANISM(S): Schizosaccharomyces pombe 
2017-06-28 | PXD005714 | Pride
Mst2 Acetylome Ms3 Acetylation Brl1 Heterochromatin 
UnknownBrr6 and Brl1 locate to nuclear pore complex assembly sites to promote their biogenesis.
Not available
S-EPMC5839787 | biostudies-literature
UnknownFunctional characterization of Pneumocystis carinii brl1 by transspecies complementation analysis.
Not available
S-EPMC2168235 | biostudies-literature
UnknownA perinuclear α-helix with amphipathic features in Brl1 promotes NPC assembly.
Not available
S-EPMC9282021 | biostudies-literature
UnknownAn amphipathic helix in Brl1 is required for nuclear pore complex biogenesis in S. cerevisiae.
Not available
S-EPMC9402233 | biostudies-literature
UnknownYeast Integral Membrane Proteins Apq12, Brl1, and Brr6 Form a Complex Important for Regulation of Membrane Homeostasis and Nuclear Pore Complex Biogenesis.
Not available
S-EPMC4664871 | biostudies-literature
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