DSS crosslinking of mLST8-CCT purified from HEK293T cells using a tandem affinity approach. First cell extract was run over HisTrap column then a streptactin column.
Temporal coordination of developmental programs is necessary for normal ontogeny, but the mechanism by which this is accomplished is poorly understood. We have previously shown that two components of the Mediator CDK8 module, CENTER CITY (CCT/MED12) and GRAND CENTRAL (GCT/MED13), are required for t...
The eukaryotic cytoplasmic chaperonin-containing TCP-1 (CCT) is a complex formed by two back-to-back stacked hetero-octameric rings that assists the folding of actins, tubulins and other proteins in an ATP-dependent manner. Here, we decided to test the significance of the hetero-oligomeric nature o...
Please provide an overall description of your study, think something similar in scope to the manuscript abstractCell proteostasiCell proteostasis includes gene transcription, protein translation, folding of de novo proteins, post-translational modification, secretion, degradation and recycling. By p...
Chemical cross-linking coupled to mass spectrometry was used to study the folding of the client protein, beta-tubulin, by the chaperonin TRiC/CCT. Different complexes containing TRiC/CCT and/or the chaperone prefoldin were cross-linked in absence or presence of nucleotides with the homobifunctional,...
