Sort   by:  
 Page size 
ProteomicsRecombinant TSP1 cleaved by HTRA1 (LCMS)
HTRA1 proteolysis of TSP1 inhibits immune suppression in age-related macular degeneration. A minor haplotype of the 10q26 locus conveys the strongest genetic risk for age related macular degeneration (AMD), a major cause of blindness affecting millions of individuals worldwide. Here we show that mo...
ORGANISM(S): Homo sapiens (Human) 
2020-07-25 | PXD020546 | Pride
Tsp1 Htra1 
ProteomicsProteomic Analysis of Human Conjunctival Cells and Tears Collected with Schirmer Strips Using TimsTOF Pro
This study aimed to investigate the human tear proteins extracted from whole (W) Schirmer strips (ScS) and their two parts, the bulb (B) and the rest of the strip (R) with a comprehensive proteomic approach using a trapped ion mobility mass spectrometer, the timsTOF Pro. Eight ScS were collected fro...
ORGANISM(S): Homo sapiens (Human) 
2022-01-27 | PXD030334 | Pride
Proteomics Conjunctival cells Schirmer strips Tears Timstof pro 
ProteomicsProtein profile of mouse ovarian follicles grown in vitro
This project presents a protein catalog of the in vitro growing mouse ovarian follicle and it provides insights about proteomics throughout in vitro follicle development.
ORGANISM(S): Mus musculus (Mouse) 
2017-10-31 | PXD006227 | Pride
Follicles Lc-msms Mouse 
ProteomicsIn-depth proteomic analysis of Plasmodium berghei sporozoites using trapped ion mobility spectrometry with parallel accumulation-serial fragmentation
We took advantage of a novel highly sensitive timsTOF PRO mass spectrometer, based on trapped ion mobility spectrometry with parallel accumulation-serial fragmentation. Combined with three alternative methods for sporozoite purification, this approach allowed us to identify the deep proteome of P. b...
ORGANISM(S): Plasmodium berghei ANKA 
2021-02-11 | PXD022735 | Pride
Malaria Mass spectrometry Sporozoites Proteome Plasmodium berghei Timstof pro 
ProteomicsIdentification of nuclear Plasmodium falciparum proteins via mass spectrometry and proposed annotation
The nuclear proteome of mixed populations of Plasmodium falciparum was explored by GeLC-MS/MS and 2D-LC-MS/MS with a peptide exclusion procedure in order to increase the detection of low abundant proteins such as those involved in gene expression.
ORGANISM(S): Plasmodium falciparum (isolate 3D7) 
2019-04-02 | PXD010427 | Pride
Plasmodium nucleus proteins proteome annotation shotgun lc-ms/ms 
ProteomicsThe MPK8-TCP14 pathway promotes seed germination in Arabidopsis
Accurate control of dormancy release and germination is critical for successful plantlet establishment. In this study, we characterized mutants of Arabidopsis MAP kinase 8 (MPK8) presenting altered dormancy. We identified Teosinte Branched1/Cycloidea/Proliferating cell factor 14 (TCP14), a key trans...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2019-07-25 | PXD013544 | Pride
Dormancy Arabidopsis Seed Phosphorylation Germination Lc-ms/ms 
ProteomicsPlasmodium sporozoites require the protein B9 to invade hepatocytes
Here we report that the B9 protein, a member of the 6-cysteine domain protein family, is secreted from sporozoite micronemes and is required for productive invasion of hepatocytes.
ORGANISM(S): Homo sapiens (Human) Plasmodium berghei 
2023-03-11 | PXD034830 | Pride
Malaria B9 protein Hepatocyte Beta-propeller domain. Invasion Plasmodium sporozoites 
ProteomicsThe AMA1-RON complex drives Plasmodium sporozoite invasion in the mosquito and mammalian hosts
Sporozoites and merozoites share a number of proteins that are expressed by both stages, including the Apical Membrane Antigen 1 (AMA1) and the Rhoptry Neck Proteins (RONs). Although AMA1 and RONs are essential for merozoite invasion of erythrocytes during asexual blood stage replication of the mala...
ORGANISM(S): Plasmodium berghei ANKA 
2022-08-12 | PXD031463 | Pride
Plasmodium Malaria Rons. Sporozoites Ama1 
ProteomicsThe claudin-like apicomplexan microneme protein is essential for Plasmodium berghei sporozoite infectivity in the mosquito and mammalian hosts
Here we characterize the function of a microneme protein expressed both in merozoites and sporozoites, the claudin-like protein CLAMP. We used a conditional genome editing strategy in a rodent malaria model to generate CLAMP-deficient sporozoites. In the absence of CLAMP, sporozoites failed to invad...
ORGANISM(S): Plasmodium berghei ANKA 
2023-05-10 | PXD037561 | Pride
Plasmodium  malaria  sporozoites  conditional mutagenesis  clamp 
Sort   by:  
 Page size