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OtherThe SILAC fly allows for accurate protein quantification in vivo.
Data from ProteomeXchange, PXD ID: PXD003040. File: Bert_20090626_JC_tud_M_standard_slice_11_2.mzml. Published as part of Mol Cell Proteomics. 2010 Oct;9(10):2173-83 . From the Abstract: {{i}} Stable isotope labeling by amino acids in cell culture (SILAC) is widely used to quantify protein abundanc...
ORGANISM(S): Dmelanogaster 
GPM32320019419 | GPMDB
Resource Reanalysis 
ProteomicsDYRK3 protein-protein interactions in human cells
Quantitative affinity purification experiment was performed to identify the protein interactors of DYRK3 kinase in human cells. An additional pull-down experiment was carried out to study the changes of DYRK3 interactors with GSK-626616 kinase inhibitor treatment.
ORGANISM(S): Homo sapiens (Human) 
2018-07-05 | PXD007761 | Pride
Ap-ms Dyrk3 Silac Kinase inhibitor 
ProteomicsThe SILAC fly allows for accurate protein quantification in vivo
Stable isotope labeling by amino acids in cell culture (SILAC) is widely used to quantify protein abundance in tissue culture cells. Until now, the only multicellular organism completely labeled at the amino acid level was the laboratory mouse. The fruit fly Drosophila melanogaster is one of the mos...
ORGANISM(S): Drosophila melanogaster (Fruit fly) 
2015-10-12 | PXD003040 | Pride
Silac In vivo Drosophila 
ProteomicsPericentriolar satellite proteome (SILAC IP)
SILAC quantitative affinity purification experiment was performed to compare the protein composition of pericentriolar satellites in the wild-type and acentrosomal (stil KO) DT40 cell lines.
ORGANISM(S): Gallus gallus (Chicken) 
2019-05-30 | PXD011247 | Pride
Ap-ms G. gallus Silac Pericentriolar satellite Pcm1 Dt40 
ProteomicsDT40 whole cell proteome (SILAC)
SILAC quantitative proteomics was used to compare the whole cell proteomes of the differentially SILAC-labelled wild-type and acentrosomal (stil KO) DT40 cell lines.
ORGANISM(S): Gallus gallus (Chicken) 
2019-05-30 | PXD011248 | Pride
G. gallus Silac Dt40 
ProteomicsPericentriolar satellite proteome
Label-free quantitative affinity purification experiments were performed to identify the protein composition of pericentriolar satellites in the wild-type and two acentrosomal (stil KO; cep152 KO) DT40 cell lines.
ORGANISM(S): Gallus gallus (Chicken) 
2019-05-30 | PXD010325 | Pride
Ap-ms G. gallus Lfq Pericentriolar satellite Pcm1 Dt40 
ProteomicsFungus-host protein-protein interactions between Drechmeria coniospora and Caenorhabditis elegans
Label-free quantitative affinity purification experiments were performed to identify the host protein binders of D. coniospora fungal virulence factors in C. elegans.
ORGANISM(S): Caenorhabditis elegans 
2020-11-20 | PXD021929 | Pride
Innate immunity Ap-ms Ppi C. elegans Drechmeria coniospora Fungus-host interaction 
ProteomicsIn vivo protein-protein interaction in C. elegans early embryos
We combine in vivo expressed GFP-tagged proteins with quantitative proteomics to identify protein-protein interactions of selected key proteins involved in early C. elegans embryogenesis. Co-affinity purification of interaction partners for eight bait proteins resulted in a pilot in vivo interaction...
ORGANISM(S): Caenorhabditis elegans 
2016-02-29 | PXD002624 | Pride
C. elegans 
ProteomicsAKIR-1 protein-protein interactions in C. elegans
We combined transgenic AKIR-1::GFP C. elegans strain and label-free quantitative affinity purification to identify protein interaction partners of AKIR-1. An additonal pull-down experiment was performed to study AKIR-1 interactions under Drechmeria coniospora infection.
ORGANISM(S): Caenorhabditis elegans 
2018-06-19 | PXD008074 | Pride
C. elegans Akir-1 Ppi 
ProteomicsProteome changes of fibroblasts and endothelial cells upon incubation with human cytomegalovirus dense bodies
A mass spectrometry-based proteomics analysis was performed to study the proteome changes upon treatment with dense bodies derived from human cytomegalovirus infection.
ORGANISM(S): Homo sapiens (Human) 
2023-07-24 | PXD039032 | Pride
Hcmv Endothelial cells Dimethyl-labelling Dense bodies Fibroblasts 
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