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MetabolomicsMechanistic insights into the biological activity of S-Sulfocysteine in CHO cells using a multi-omics approach
S-Sulfocysteine (SSC), a bioavailable L-cysteine derivative (Cys), is known to be taken up and metabolized in Chinese hamster ovary (CHO) cells used to produce novel therapeutic biological entities. To gain a deeper mechanistic insight into the SSC biological activity and metabolization, a multi-omi...
2024-08-08 | MTBLS7424 | MetaboLights
MetabolomicsWhat CHO is made of: Variations in the biomass composition of Chinese hamster ovary cell lines

Background: Cell line specific, genome-scale metabolic models enable rigorous and systematic in silico investigation of cellular metabolism. Such models have recently become available for Chinese hamster ovary (CHO) too. However, a key ingredient, namely an experimentally validat...

2020-07-13 | MTBLS1210 | MetaboLights
MetabolomicsAn LC–MS-based lipidomics pre-processing framework underpins rapid hypothesis generation towards CHO systems biotechnology

INTRODUCTION: Given a raw LC–MS dataset, it is often required to rapidly generate initial hypotheses, in conjunction with other ‘omics’ datasets, without time-consuming lipid verifications. Furthermore, for meta-analysis of many datasets, it may be impractical to conduct exhausti...

2018-07-17 | MTBLS493 | MetaboLights
TranscriptomicsCHO cells with enhanced and abolished gamma-secretase activity
γ-secretase is an intra-membrane-cleaving aspartyl protease implicated in the processing of a wide range of type I membrane proteins including the Notch receptor and the amyloid-β precursor protein (APP). It thus regulates a diverse array of cellular and biological processes including the differen...
ORGANISM(S): Cricetulus griseus 
2010-05-15 | E-GEOD-16379 | biostudies-arrayexpress
TranscriptomicsAnalysis of mature miRNA levels in the context of CHO cell proliferation
Mature microRNA transcripts were analyzed in 9 CHO cell lines growing at different proliferation rates. The goal was to identify microRNAs that correlate positively or negatively with CHO proliferation rates. A common reference design was chosen, where all samples were hybridized against a pool of a...
ORGANISM(S): Cricetulus griseus 
2015-12-10 | E-GEOD-75830 | biostudies-arrayexpress
ProteomicsComparative proteomics profiling of CHO and fast-growing CHL-YN cells
Comprehensive identification of activated biological processes and pathways in Chinese hamster ovary (CHO) cells and Chinese hamster lung (CHL)-YN cells by proteome profiling, with a focus on their functional characteristics as host cells for biopharmaceutical production. In CHL-YN cells, processes ...
ORGANISM(S): Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) 
2025-10-03 | PXD066507 | Pride
Chinese hamster lung cells Proteomics Chinese hamster ovary cells 
TranscriptomicsRNA-seq of miRNA and piRNA of Exosomes and whole cell lysates from CHO-K1 Cell Cultures at different growth phases
In biopharmaceutical production, Chinese hamster ovary (CHO) cells derived from Cricetulus griseus remain the most commonly used host cell for recombinant protein production, especially antibodies. Over the last decade in-depth multi-omics characterization of these CHO cells provided data for extens...
ORGANISM(S): Cricetulus griseus 
2021-01-18 | E-MTAB-9894 | biostudies-arrayexpress
ProteomicsChinese hamster ovary (CHO) cell Surfaceome LC-MSMS and mRNA expression analysis
The production of biopharmaceuticals relies on robust cell systems that can produce recombinant proteins at high levels, and grow and survive in the stressful bioprocess environment. Chinese hamster ovary cells (CHO) as the main production hosts offer a variety of advantages including robust growth ...
ORGANISM(S): Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) 
2022-10-15 | PXD033581 | Pride
Lc-msms Signaling pathways Mrna expression Cho cells Surfaceome 
ProteomicsHyperosmolality in CHO Culture: Effects on Proteome
Chinese hamster ovary cells (CHO) were exposed to highly concentrated feed solution during a fed-batch cultivation which causes an unphysiological osmolality increase (>300 mOsm/kg) affecting cell physiology, morphology and proteome. To get a deeper insight into underlying molecular mechanisms invol...
ORGANISM(S): Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) 
2022-05-20 | PXD027443 | Pride
Nlc-ms lfq proteomics Fed-batch Q exactive plus Cho Hyperosmolality Cell size 
ProteomicsQuantitative Proteomics Reveals Cellular Responses to Stress and Tunicamycin in CHO Cells
We conducted proteomics analysis aimed at evaluating the differences between CHO cell lines expressing 2G12 and 353/11, cells were cultured using a semi-perfusion batch process in parallel. The cells were collected and pelleted on day six, four hours after medium exchange, when both cell lines had r...
ORGANISM(S): Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) 
2024-07-03 | PXD049297 | Pride
Ms/ms Cho Lfq Orbitrap q exactive Proteomics 
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