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MetabolomicsMechanistic insights into the biological activity of S-Sulfocysteine in CHO cells using a multi-omics approach
S-Sulfocysteine (SSC), a bioavailable L-cysteine derivative (Cys), is known to be taken up and metabolized in Chinese hamster ovary (CHO) cells used to produce novel therapeutic biological entities. To gain a deeper mechanistic insight into the SSC biological activity and metabolization, a multi-omi...
2024-08-08 | MTBLS7424 | MetaboLights
ProteomicsQuantitative analysis of the supernatant from native and transfected CHO cells using iTRAQ 8-plex technique
We employed UPLC-MS/MS with iTRAQ 8-plex labeling to quantitatively analyze the supernatant produced by two Chinese hamster ovary (CHO) cell lines (CHO K1SV and CHO CAT-S). In each case, the supernatant from the host and three transfected clones were analyzed at days 5, 7, and 10 of culture. A total...
ORGANISM(S): Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) 
2016-07-20 | PXD003462 | Pride
Cho supernatant Itraq 8-plex Lc-ms/ms 
TranscriptomicsGene expression profiles in semi-continuous culture with high-cell density under normal and low temperature conditions for scFv-Fc-producer CHO cells harboring an artificial promoter system with transcriptional positive-feedback loop of transactivator
We developed an artificial gene expression system with transcriptional positive-feedback loop of transactivator to induce the high expression of target genes in CHO cells. DNA microarray analysis was conducted in semi-continuous culture with high-cell-density culture under normal and low temperature...
ORGANISM(S): Cricetulus griseus 
2024-01-01 | E-MTAB-13440 | biostudies-arrayexpress
ProteomicsGlycoprofile Comparison of SARS-CoV-2 Spike Proteins Expressed in CHO and HEK Cell Lines
This study assessed the N-glycosylation profiles of a full-length trimeric spike protein expressed in either HEK or CHO cells. Glycopeptide analysis was performed using a tandem mass spectrometry workflow and BioPharma Finder™ using HEK and CHO glycan databases for protein characterisation. The resu...
ORGANISM(S): Homo sapiens (Human) 
2025-09-15 | PXD054718 | Pride
Covid-19 Hek cells Ms-ms Sars-cov-2 N-glycosylation Cho cells 
TranscriptomicsRNA-seq of miRNA and piRNA of Exosomes and whole cell lysates from CHO-K1 Cell Cultures at different growth phases
In biopharmaceutical production, Chinese hamster ovary (CHO) cells derived from Cricetulus griseus remain the most commonly used host cell for recombinant protein production, especially antibodies. Over the last decade in-depth multi-omics characterization of these CHO cells provided data for extens...
ORGANISM(S): Cricetulus griseus 
2021-01-18 | E-MTAB-9894 | biostudies-arrayexpress
ProteomicsHyperosmolality in CHO Culture: Effects on Proteome
Chinese hamster ovary cells (CHO) were exposed to highly concentrated feed solution during a fed-batch cultivation which causes an unphysiological osmolality increase (>300 mOsm/kg) affecting cell physiology, morphology and proteome. To get a deeper insight into underlying molecular mechanisms invol...
ORGANISM(S): Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) 
2022-05-20 | PXD027443 | Pride
Nlc-ms lfq proteomics Fed-batch Q exactive plus Cho Hyperosmolality Cell size 
ProteomicsSpatial proteomics reveals differences in the cellular architecture of antibody-producing CHO and plasma-cell derived cells
Most of the recombinant biotherapeutics employed today to combat severe illnesses e.g. various types of cancer or autoimmune diseases are produced by Chinese hamster ovary (CHO) cells. To meet the growing demand of these pharmaceuticals, CHO cells are under constant development in order to enhance t...
ORGANISM(S): Mus musculus (Mouse) Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) 
2022-08-02 | PXD029115 | Pride
Machine learning Chinese hamster ovary Subcellular proteomics Mass spectrometry Myeloma 
UnknownElucidation of the CHO Super-Ome (CHO-SO) by Proteoinformatics.
Not available
S-EPMC4721571 | biostudies-literature
ProteomicsDetermination of GPI structure of human CD59 in various KO CHO cells
To determine GPI structure of human CD59 in PGAP4-KO, PGAP3-KO, PGAP5-KO, and PGAP2-KO CHO cells.
ORGANISM(S): Homo sapiens (Human) 
2018-01-30 | PXD008230 | Pride
Lc-msms Cd59 Pgap5 Pgap4 Cho cells Pgap3 Gpi Pgap2 
ProteomicsIsolation and Characterization of small EVs from CHO-K1 cell cultures
Chinese hamster ovary (CHO) cells are widely used host cells for recombinant protein production and currently the most commonly utilized mammalian organism in large scale biopharmaceutical production. Since the discovery of exosomes as a new type of small extracellular vesicles (EVs) by Johnstone et...
ORGANISM(S): Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) 
2021-09-09 | PXD018446 | Pride
Proteomics Cho Cell culture Extracellular vesicles Exosomes 
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