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ProteomicsCharacterisation of nuclear and sub-nuclear proteomes of Saccharomyces cerevisiae
This study concerns characterisation of the nuclear, chromatin and nuclear non-chromatin proteomes of the yeast Saccharomyces cerevisiae. We performed nuclear and chromatin enrichments from exponentially growing cells using differential centrifugation and analysed the nuclear and chromatin pellets, ...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2019-11-07 | PXD007525 | Pride
Shotgun proteomics Organelle Nucleus Saccharomyces cerevisiae Chromatin Lc-ms/ms 
UnknownInhibition of transcription leads to rewiring of locus-specific chromatin proteomes.
Not available
S-EPMC7197482 | biostudies-literature
UnknownDistinct and diverse chromatin proteomes of ageing mouse organs reveal protein signatures that correlate with physiological functions.
Not available
S-EPMC8933006 | biostudies-literature
ProteomicsComprehensive chromatin proteomics resolves functional phases of pluripotency and identifies changes in regulatory components
The establishment of cellular identity is driven by transcriptional and epigenetic regulators of the chromatin proteome - the chromatome. Comprehensive analyses of the chromatome composition and dynamics can therefore greatly improve our understanding of gene regulatory mechanisms. Here, we develope...
ORGANISM(S): Mus musculus (Mouse) Homo sapiens (Human) 
2023-02-27 | PXD034448 | Pride
Naive Dia Pluripotency Mass spectrometry Stem cells Formative Chromatin proteomics Primed 
ProteomicsBridgin connects the outer kinetochore to centromeric chromatin
In this project, 3XFlag-tagged protein affinity purifications were performed to identify the protein-protein interactors using Cryptococcus neoformans cell lysate. After fractionation with SDS-PAGE, peptide samples for nanoLC-MS/MS were prepared by in-gel digestion. Raw data from Orbotrap Velos pr...
ORGANISM(S): Cryptococcus Neoformans 
2020-09-23 | PXD021072 | JPOST Repository
ProteomicsBANC-seq to identify genome-wide transcription factor binding affinities to native chromatin
BANC-seq (Binding Affinities to Native Chromatin by sequencing) allows determination of absolute apparent binding affinites of transcription factors to native chromatin in a genome-wide manner. In this study, we establish the method and show that chromatin and DNA sequence define binding affinites o...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2023-02-09 | PXD038502 | Pride
Dna pulldowns Transcription factors Tmt Proteomes Binding affinity Absolute quantification 
ProteomicsMudPIT analyses of the chromatin associated proteins isolated from the nucleus of Plasmodium falciparum at three time-points of the intraerythrocytic development cycle
Parasite cultures- Plasmodium falciparum strain 3D7 was cultured in human O+ erythrocytes at 5% hematocrit. Cultures were synchronized at ring stage with 5% (w/v) D-sorbitol treatments. Parasite cultures (8% parasitemia in 5% hematocrit) were harvested 48 hours after the first sorbitol treatment (ri...
ORGANISM(S): Plasmodium Falciparum 3d7 (ncbitaxon:36329) 
2018-06-28 | MSV000082520 | MassIVE
ProteomicsFGF2 antiproliferative stimulus triggers proteomic and transcriptional changes associated with nucleolar disorganization in K-ras-driven mouse tumor cells (Chromatin MudPIT data)
Chromatin plays a crucial role in the intermediation between cell signaling and gene expression. The nucleolus is sensitive to stress and can orchestrate a chain of cellular events in response to stress signals. Despite being a growth factor, FGF2 has anti-proliferative and tumor-suppressive functio...
ORGANISM(S): Mus Musculus (ncbitaxon:10090) 
2022-10-11 | MSV000090511 | MassIVE
ProteomicsProteomes of chromatin fractions derived from SU-DHL-5 cells
SU-DHL-5 cells display limited expression of the SUMO isopeptidase SENP6. In this experiment, the chromatin associated fraction of SU-DHL-5 cells was analysed by mass spectrometry. SU-DHL-5 cells were either stably transfected with an empty vector or with a SENP6 expression construct. Changes in pro...
ORGANISM(S): Homo sapiens (Human) 
2022-02-17 | PXD027355 | Pride
Su-dhl-5 cells (b-cell lymphoma) 
ProteomicsChromatin Proteome of Drosophila Ovarian somatic cells
Chromatin Proteome was enriched from drosophila ovarian somatic cells using ChEP.
ORGANISM(S): Drosophila Melanogaster (fruit Fly) 
2021-07-02 | PXD024167 | JPOST Repository
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