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ProteomicsClick-linking: a cell-compatible protein crosslinking method based on click chemistry
We developed a novel approach to in situ crosslinking mass spectrometry (XL-MS) where the reaction is split into two sequential and orthogonal coupling events. The method involves pre-stabilization of the proteome using a fixation protocol, followed by a two-step process that begins with extensive l...
ORGANISM(S): Homo sapiens (Human) Escherichia coli 
2025-01-28 | PXD059118 | Pride
In situ Crosslinking Proteome-wide Protein-protein interactions Interactome 
GenomicsIdentification of newly synthesized proteins in microbial communities using BONCAT and click chemistry
Identification of newly synthesized proteins in microbial communities using BONCAT and click chemistry
PRJEB70937 | ENA
ProteomicsBroadening the Utility of Farnesyltransferase-Catalyzed Protein Labeling Using Norbornene–Tetrazine Click Chemistry
Bio-orthogonal chemistry has gained widespread use in the study of many biological systems of interest, including protein prenylation. Prenylation is a post-translational modification in which a 15 or 20 carbon isoprenoid chain is transferred onto a cysteine near the C-terminus of a target protein. ...
ORGANISM(S): Homo sapiens (Human) 
2024-05-21 | PXD045277 | Pride
Prenylation Lfq Click chemistry 
ProteomicsIdentification of Cytosolic Protein Targets of Catechol Estrogens in Breast Cancer Cells Using a Click Chemistry-based Workflow
Catechol estrogens (CEs) are known to be toxic metabolites and the initiators in the oncogenesis of breast cancers via forming covalent adducts with DNAs. CEs shall also react with proteins but their cellular protein targets remain un-explored. Here we reported the identification of protein targets ...
ORGANISM(S): Homo sapiens (Human) 
2020-09-28 | PXD017731 | Pride
Affinity purification Catechol estrogens Breast cancer cells Mass spectrometry Proteomic 
ProteomicsTracing active members in microbial communities by BONCAT and click chemistry-based enrichment of newly synthesised proteins
Metaproteomics enables the description of microbial communities (MC). Microbial adaptation to changing environments is conducted by expressing newly synthesized proteins (nP) that can be difficult to distinguish from background proteins. Focusing on nP would add a new dimension to the metaproteomics...
ORGANISM(S): Escherichia coli (strain K12 / W3110 / ATCC 27325 / DSM 5911) Saccharomyces cerevisiae (Baker's yeast) anaerobic digester metagenome 
2024-10-17 | PXD047252 | Pride
Boncat Anaerobic digestion Metaproteomics Microbiome Mass spectrometry Click chemistry 
ProteomicsEnhanced protein-protein interaction network construction promoted by in vivo cross-linking with acid-cleavable click chemistry enrichment
Chemical cross-linking coupled with mass spectrometry has emerged as a powerful strategy which enables global profiling of protein interactome with direct interaction interfaces in complex biological systems. The alkyne-tagged enrichable cross-linkers are preferred to improve the coverage of low-abu...
ORGANISM(S): Homo Sapiens (human) 
2022-07-14 | PXD035317 | JPOST Repository
ProteomicsIn-situ Click Reaction Coupled with Quantitative Proteomics for Identification of Protein Targets of Catechol Estrogens
Catechol estrogens (CEs) are metabolic electrophiles that actively undergo covalent interaction with cellular proteins, influencing molecular function. There is no feasible method to identify their binders in a living system. Herein, we developed a click chemistry-based approach using ethinylestradi...
ORGANISM(S): Rattus norvegicus (Rat) 
2022-02-28 | PXD009657 | Pride
Post translational modification Metabolites Proteomics Electrophiles Dimethyl labeling Lc/ms Catechol estrogens Oxidative stress Click chemistry Redox modification 
GenomicsClick chemistry enables comprehensive preclinical evaluation of targeted epigenetic therapies [Click-Seq 2]
The success of targeted therapies hinges on our ability to understand the molecular and cellular mechanism of action of these agents. Here we modify various BET bromodomain inhibitors, an exemplar novel targeted therapy, to create functionally conserved compounds that are amenable to click-chemistry...
ORGANISM(S): Homo sapiens 
2017-07-13 | GSE88749 | GEO
GenomicsClick chemistry enables comprehensive preclinical evaluation of targeted epigenetic therapies [Click-Seq 1]
The success of targeted therapies hinges on our ability to understand the molecular and cellular mechanism of action of these agents. Here we modify various BET bromodomain inhibitors, an exemplar novel targeted therapy, to create functionally conserved compounds that are amenable to click-chemistry...
ORGANISM(S): Homo sapiens 
2017-07-13 | GSE88748 | GEO
ProteomicsClick Chemistry-based Thiol Redox Proteomics Reveals Significant Cysteine Reduction Induced by Chronic Ethanol Consumption
In the U.S., alcohol-associated liver disease (ALD) impacts millions of people and is a major healthcare burden. While the pathology of ALD is unmistakable, the molecular mechanisms underlying ethanol hepatotoxicity are not fully understood. Hepatic ethanol metabolism is intimately linked with alte...
ORGANISM(S): Mus musculus (Mouse) 
2024-09-19 | PXD042070 | Pride
Redox proteomics Proteomics Glutathione Mus musculus Agilent 6550 q-tof Redox Cysteine Thiol Click chemistry Alcohol-associated liver disease 
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