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TranscriptomicsRIP-Chip of endogenous Staufen and GFP-tagged Staufen from 0-3 hour Drosophila embryos
Staufen-associated mRNAs were identified in early Drosophila embryos by RNA-co-immunoprecipitation followed by microarray analysis (RIP-Chip), using two complementary approaches: RIP-Chip from wild-type embryos using a synthetic anti-Staufen antibody, and RIP-Chip from transgenic GFP-Staufen-express...
ORGANISM(S): Drosophila melanogaster 
2013-09-05 | E-GEOD-43418 | biostudies-arrayexpress
ProteomicsLINE-1 ORF1p co-immunoprecipitations analyzed by DDA and two DIA methods
Using an anti-ORF1 monoclonal (clone 4H1; Milipore), four co-immunoprecipitations to enrich for LINE-1 ORF1p and its interactors as described in Di Stefano, L.H. et al. (https://doi.org/10.1007/978-1-0716-2883-6_12), from either HEK-293TLD (cells transfected with pMT646- containing a L1 sequence) or...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2024-07-24 | MSV000095427 | MassIVE
TranscriptomicsRNA co-immunoprecipitations with yeast She proteins
RNA co-immunoprecipitations with C-terminal protein A-tagged She proteins. One liter of cells were cultured at 30 degrees in YPAD medium and collected during exponential growth by centrifugation. Cells were washed twice and broken mechanically with glass beads. Extracts were incubated with IgG-agaro...
ORGANISM(S): Saccharomyces cerevisiae 
2005-12-21 | GSE3879 | GEO
TranscriptomicsRNA co-immunoprecipitations with yeast She proteins
RNA co-immunoprecipitations with C-terminal protein A-tagged She proteins. One liter of cells were cultured at 30 degrees in YPAD medium and collected during exponential growth by centrifugation. Cells were washed twice and broken mechanically with glass beads. Extracts were incubated with IgG-agar...
ORGANISM(S): Saccharomyces cerevisiae 
2005-12-20 | E-GEOD-3879 | biostudies-arrayexpress
GenomicsSaccharomyces cerevisiae
RNA co-immunoprecipitations with yeast She proteins
PRJNA94119 | ENA
ProteomicsThe HAPSTR2 retrogene buffers stress signaling and resilience in mammals
Standard anti-FLAG magnetic beads for co-immunoprecipitations, elution in 2x SDS sample buffer, loaded into a stacking gel and then in-gel digested
ORGANISM(S): Homo sapiens (Human) 
2023-03-11 | PXD038642 | Pride
Hapstr2 Hapstr1 Huwe1 
ProteomicsSpodoptera frugiperda kinetochore co-immunoprecipitations
Accurate chromosome segregation requires assembly of the multiprotein kinetochore complex at centromeres. In most eukaryotes, kinetochore assembly is primed by the histone H3 variant CenH3, which physically interacts with components of the inner kinetochore constitutive-centromere-associated-network...
ORGANISM(S): Spodoptera frugiperda Bombyx mori (Silk moth) 
2020-02-18 | PXD016092 | Pride
Bombix Kinetochore Frugiperda Spodoptera 
ProteomicsDiscovery of Klf2 Interactors in Mouse Embryonic Stem Cells by Immunoprecipitation-Mass Spectrometry Utilizing Exogenously Expressed Bait
Krüppel-like factor 2 (Klf2) is a DNA-binding transcription factor that regulates embryonic stem cell-specific gene expression. Transcription cofactors such as p300 acetyltransferases and Erk kinases interact with Klf2, providing an additional layer of transcription regulation in embryonic stem cell...
ORGANISM(S): Mus Musculus (mouse) 
2021-09-20 | PXD022123 | JPOST Repository
OtherAssessment of a method to characterize antibody selectivity and specificity for use in immunoprecipitation.
Data from ProteomeXchange, PXD ID: PXD002534. File: GR_130129_20_OT3_NT_L3MBTL2_FAB_31_G11_2_HEK293.mzXML. Published as part of Nat Methods. 2015 Jun 29 . From the Abstract: {{i}} ... We describe a mass spectrometry-based standard operating procedure for scoring immunoprecipitation antibody quality...
ORGANISM(S): Homo_sapiens_viruses, Human_female 
GPM32320016573 | GPMDB
Resource Reanalysis 
TranscriptomicsmRNA composition of IRP2 mRNPs in mouse tissues
Affymetrix microarrays were used to determine the mRNA composition of mRNPs obtained by immunoprecipitation with IRP2 (iron regulatory protein 2). Reference Sanchez at al., 2007 Nat. Protoc. Affymetrix microarrays were used to determine the mRNA composition of mRNPs obtained by immunoprecipitation ...
ORGANISM(S): Mus musculus 
2011-12-09 | E-GEOD-17097 | biostudies-arrayexpress
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