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ProteomicsThe interactome of flavin adenine dinucleotide (FAD) with RNA-binding proteins
We explored the interactome of flavin adenine dinucleotide (FAD) and the matrix of UV-immobilised FAD bound more than 3,000 proteins from SW-620 lysate. GO enrichment analysis showed that about 600 RNA-binding proteins were bound to FAD beads and, surprisingly, more than 40 proteins showed clear dos...
ORGANISM(S): Homo sapiens (Human) 
2022-10-06 | PXD036953 | Pride
Pum-rna Photo-immobilisation Fad Competition pulldown 
ProteomicsDiazirine photo-immobilisation for target profiling of natural products and co-factors: pulldown data
Finding the targets of natural products is of key importance in both chemical biology and drug discovery, and deconvolution of cofactor interactomes contribute to the functional annotation of the proteome. Identifying the proteins that underlie natural compound activity in phenotypic screens help to...
ORGANISM(S): Homo sapiens (Human) Escherichia coli 
2022-10-06 | PXD033292 | Pride
Photo-immobilisation Pulldowns Natural products profiling Natural products targets 
UnknownDevelopment of substrate-selective probes for affinity pulldown of histone demethylases.
Not available
S-EPMC4301071 | biostudies-literature
ProteomicsAza-SAHA Derivatives are Selective Histone Deacetylase 10 Chemical Probes That Inhibit Polyamine Deacetylation
We report the first selective chemical probes for histone deacetylase 10 (HDAC10) with unprecedented selectivity over other HDAC isozymes. HDAC10 deacetylates polyamines and has a distinct substrate specificity, making it unique among the 11 zinc-dependent HDAC hydrolases. Taking inspiration from HD...
ORGANISM(S): Homo sapiens (Human) 
2026-06-02 | PXD032671 | Pride
Histone deacetylase Polyamines Be(2)-c Drug discovery Chemical proteomics Chemical biology Chemical probes Lc-ms/ms 
ProteomicsSchwalm et al_2023_Targeting LC3/GABARAP for degrader development and autophagy modulation
Chemoproteomic competition pulldown assays with immobilized drug molecules and free drug molecules in cell lysates.
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2023-11-28 | MSV000093528 | MassIVE
ProteomicsShotgun Proteomic Identification of Amphotericin B Targets in Late Endosomes of Influenza A Virus-Infected A549 Cells
Amphotericin B (AmB), a polyene macrolide antifungal, is well known for its ability to bind ergosterol and cholesterol; however, its interactions with host proteins remain poorly characterized. In this study, we aimed to identify AmB-binding targets in A549 cells infected with the influenza A virus ...
ORGANISM(S): Homo sapiens (Human) 
2026-02-02 | PXD064601 | Pride
Amphotericin b  influenza a virus  late endosome  shotgun proteomics 
ProteomicsDifferential Chemoproteomics Reveals MARK2/3 as Cell Migration-Relevant Targets of the ALK Inhibitor Brigatinib
Metastasis poses a major challenge in cancer management, including EML4-ALK-rearranged non-small cell lung cancer (NSCLC). As cell migration is a critical step during metastasis, we assessed the anti-migratory activities of several clinical ALK inhibitors in NSCLC cells and observed differential ant...
ORGANISM(S): Homo sapiens (Human) 
2024-04-05 | PXD036716 | Pride
Anaplastic lymphoma kinase Lung cancer Brigatinib Chemical proteomics Alk 
UnknownCompetition of calcified calmodulin N lobe and PIP2 to an LQT mutation site in Kv7.1 channel.
Not available
S-EPMC5293103 | biostudies-literature
ProteomicsQuantification and discovery of sequence determinants of protein per mRNA amount in 29 human tissues
We analyzed the contribution of known sequence determinants of protein synthesis and degradation and novel mRNA and protein sequence motifs that we found by association testing. In order to investigate the functional understanding of novel mRNA motifs, an RNA competitive-binding assay was developed ...
ORGANISM(S): Homo sapiens (Human) 
2019-02-20 | PXD010153 | Pride
Rna pulldown 
ProteomicsThe non-canonical PARP1 inhibitor target, PARP16, contributes to talazoparib polypharmacology and synergy with WEE1 inhibitors: Chemical Proteomics Cal51
PARP1 inhibitors (PARP1is) display single-agent anticancer activity in small cell lung cancer (SCLC) and other neuroendocrine tumors independent of BRCA1/2 mutations. Here, we determined the differential efficacy of multiple clinical PARP1is in SCLC cells. Compared to the other PARP1is (rucaparib, o...
ORGANISM(S): Homo sapiens (Human) 
2021-07-21 | PXD020520 | Pride
Adavosertib Parp16 Breast cancer Parp1 Polypharmacology Parp inhibitor Proteomics Off-target Talazoparib 
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