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GenomicsmiR-206 pulldowns in FAPs to idenfity transcript targets
miR-206 pulldowns in FAPs to idenfity transcript targets
PRJNA719821 | ENA
ProteomicsC-Man peptide pulldowns from Brain
Peptide pulldowns with C-man antibodies to demostrate the utelity of reagents for proteomic analysis. Using Murine brain samples Novel C-man sites are readily identifiable.
ORGANISM(S): Mus musculus (Mouse) 
2020-11-16 | PXD020336 | Pride
C-man Affinity pulldowns Glycopeptides 
ProteomicsSvf1-GFP pulldowns from Limar et al. 2023 JCB
Svf1-GFP pulldowns compared to untegged WT cells from SILAC labelled yeast cells. See Limar et al. 2023 JCB
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2023-03-06 | PXD039395 | Pride
Svf1-gfp pulldowns from yeast svf1 
ProteomicsPull-downs of non-crosslinked or in-cell SDA crosslinked FLAG-AK2-transfected 293 cells
Human cells were transfected with a plasmid encoding FLAG-AK2 and subsequently treated with a photoactivatable crosslinker, SDA, or left untreated. Pulldowns were performed from these cells, and the bead-bound material was digested and subjected to LC-MS DIA runs for analysis.
ORGANISM(S): Homo sapiens (Human) 
2025-05-06 | PXD052867 | Pride
Dia Crossslinking Lc-ms Pulldowns Transfection Sda 
ProteomicsBANC-seq to identify genome-wide transcription factor binding affinities to native chromatin
BANC-seq (Binding Affinities to Native Chromatin by sequencing) allows determination of absolute apparent binding affinites of transcription factors to native chromatin in a genome-wide manner. In this study, we establish the method and show that chromatin and DNA sequence define binding affinites o...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2023-02-09 | PXD038502 | Pride
Dna pulldowns Transcription factors Tmt Proteomes Binding affinity Absolute quantification 
ProteomicsChemoproteomics of an NSD2-PWWP1 Chemical Probe
Here we use competitive chemoproteomics pulldowns followed by label-free quantitative LC-MS/MS to assess target engagement and selectivity profiles of UNC6934 and UNC7145, a chemical probe targeting the PWWP1 domain of NSD2 and its negative control counterpart, respectively. To this end, we used a b...
ORGANISM(S): Homo sapiens (Human) 
2021-08-06 | PXD017641 | Pride
Epigenetics Chemoproteomics Histone methyltransferase Chemical probe 
ProteomicsEvaluation of peptide pulldown coupled to mass spectrometry approaches for investigation of motif-based protein-protein interactions
Eight different, SLiM-containing peptides were tested for pulldowns immobilized on magnetic beads, and the same sequences were used for PRISMA experiments. Double repeat of peptides were also used for higher local concentration of the motifs.
ORGANISM(S): Homo sapiens (Human) 
2023-03-07 | PXD037380 | Pride
Ap-ms Prisma Pulldown Slim Protein-protein interaction 
ProteomicsLimited-proteolysis coupled to mass spectrometry (LiP-MS) data on a serially filtered yeast lysate (the FLiP library) and on lysates of wild-type and Gcn5 mutant yeast strains grown under hydroxyurea stress and control conditions. Affinity purification mass spectrometry on Ada3 pulldowns from yeast grown under hydroxyurea stress and control conditions.
We applied serial ultrafiltration and limited proteolysis-coupled mass spectrometry (LiP-MS) to generate an atlas of peptides that act as markers for the formation or dissolution of protein-protein interactions (PPIs). Applied to Saccharomyces cerevisiae, the approach yielded 6,441 candidate PPI mar...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2024-10-17 | PXD055566 | Pride
Ap-ms Flip-ms Hydroxyurea Ada3 Lip-ms 
ProteomicsIdentification of novel RHOA interacting patners
RHO subfamily of small GTPases comprise highly conserved family members RHOA, RHOB, and RHOC which cycle between GTP-bound 'active' and GDP-bound 'inactive' states. In the active form, RHO proteins interact with a variety of downstream effector proteins, controlling their activity and function. Many...
ORGANISM(S): Homo sapiens (Human) 
2016-11-01 | PXD004934 | Pride
Silac  lc-ms  protein-protein interactions  rhoa 
OthermiR-206 pulldowns in FAPs to idenfity transcript targets
Biotin labelled miR-206 mimics ('06") and miR-Control mimics ("NC") were used to indentify putative miRNA transcript targets in FAPs form skeletal muscle
ORGANISM(S): Mus musculus 
2021-05-01 | GSE171504 | GEO
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