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ProteomicsPull-downs of non-crosslinked or in-cell SDA crosslinked FLAG-AK2-transfected 293 cells
Human cells were transfected with a plasmid encoding FLAG-AK2 and subsequently treated with a photoactivatable crosslinker, SDA, or left untreated. Pulldowns were performed from these cells, and the bead-bound material was digested and subjected to LC-MS DIA runs for analysis.
ORGANISM(S): Homo sapiens (Human) 
2025-05-06 | PXD052867 | Pride
Dia Crossslinking Lc-ms Pulldowns Transfection Sda 
ProteomicsIdentification of chromatin-associated proteins occupying the promoter region of CDKN1A
The p21 protein, encoded by CDKN1A, plays a vital role in the induction of senescence, and its transcriptional control by p53 tumour supressor is well-established. However, p21 can also be regulated in a p53-independent manner, by mechanisms that remain poorly understood. Therefore, we here used a c...
ORGANISM(S): Homo sapiens (Human) 
2021-09-07 | PXD022542 | Pride
Cdkn1a Znf84 Chromatin-associated proteins 
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