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Peptide pulldowns with C-man antibodies to demostrate the utelity of reagents for proteomic analysis. Using Murine brain samples Novel C-man sites are readily identifiable.
ORGANISM(S): Mus musculus (Mouse) 
2020-11-16 | PXD020336 | Pride
GST:Repo-Man(C-terminus) or GST:alone were expressed as in Vagnarelli et al. 2011. Before elution, beads were first incubated with nucleosomes extracted from HeLa. HeLa cells were lysed and nucleosomes were digested with MNase (20min, 37C). Nucleosomes were firstly incubated with GST alone for 2hrs....
ORGANISM(S): Homo sapiens (Human) 
2016-12-07 | PXD004613 | Pride
Actin-related proteins are ubiquitous components of chromatin remodelers, and are conserved from yeast to man. We have examined the role of the budding yeast actin-related protein Arp6 in gene expression, both as a component of the SWR1 complex (SWR-C) and in its absence. We mapped Arp6-binding site...
ORGANISM(S): Saccharomyces cerevisiae 
Clostridium botulinum is a heterogeneous Gram-positive species that comprises four genetically and physiologically distinct groups of bacteria that share the ability to produce botulinum neurotoxin, the most poisonous toxin known to man, and the causative agent of botulism, a severe disease of human...
ORGANISM(S): Clostridium botulinum 
High-throughput pyrosequencing of endogenous small RNAs from >95% male enriched populations of alg-3(tm1155);alg-4(ok1041);fog-2(q71) and fog-2(q71) worms as well as purified spermatids from fem-3(q20) adult worms. Gametogenesis is thermosensitive in numerous metazoa ranging from worms to man. In C...
ORGANISM(S): Caenorhabditis elegans 
The aim of the study was to determine biological relevance of differentially expressed genes in Lactobacillus plantarum C2 during fermentation of plant substrates. Whole-transcriptome analysis based on customized microarray profiles has been used to determine altered transcription patterns in L. pla...
ORGANISM(S): Lactobacillus plantarum 
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