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TranscriptomicsDirected shotgun proteomics guided by RNA-Seq identifies a complete expressed prokaryotic proteome
Prokaryotes are, due to their moderate complexity, particularly amenable to the comprehensive identification of the protein repertoire expressed under different conditions. We applied a generic strategy to identify a complete expressed prokaryotic proteome, which is based on the analysis of RNA and ...
ORGANISM(S): Bartonella henselae 
2013-04-23 | E-GEOD-44564 | biostudies-arrayexpress
ProteomicsMetal organic layers enabled cell surface engineering coupling biomembrane fusion for dynamic membrane proteome profiling
Systematically dissecting the highly dynamic and tightly communicating membrane proteome of living cells is essential for systems-level understanding of fundamental cellular processes and intricate relationship between membrane-bound organelles constructed through membrane traffic. While extensive e...
ORGANISM(S): Homo sapiens (Human) 
2024-01-26 | PXD044945 | Pride
Membrane protein enrichment metal organic layers proteome 
ProteomicsMembrane proteomis profile along human colon
The colonic epithelium is a highly dynamic system important for regulation of ion and water homeostasis via absorption and secretion, and in maintaining a protective barrier between the outer milieu and the inside of our body. These processes are known to gradually change along the length of the col...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2016-03-07 | MSV000079561 | MassIVE
ProteomicsComplete Expressed Proteome of Bartonella Henselae
Using a discovery proteomics approach, the expressed proteome of Bartonella henselae, a Gram-negative prokaryotic model organism was exhaustively studied under two conditions that mimic those encountered in different hosts. Using the analysis-driven experimentation (ADE) feedback-loop strategy, w...
ORGANISM(S): Bartonella henselae (strain ATCC 49882 / Houston 1) (Rochalimaea henselae) 
2013-07-18 | PXD000153 | Pride
Complete membrane proteome Bartonella henselae Complete proteome Ade 
TranscriptomicsDirected shotgun proteomics guided by RNA-Seq identifies a complete expressed prokaryotic proteome
Prokaryotes are, due to their moderate complexity, particularly amenable to the comprehensive identification of the protein repertoire expressed under different conditions. We applied a generic strategy to identify a complete expressed prokaryotic proteome, which is based on the analysis of RNA and ...
ORGANISM(S): Bartonella henselae 
2013-04-23 | GSE44564 | GEO
ProteomicsHuman_MCF7_membrane - Searching Missing Proteins Based on the Optimization of Membrane Protein Enrichment and Digestion Process
MCF7 cell was an important stabilized breast cancer cell in cancer research. A membrane proteomic method was developed and compared in this study. A membrane protein enrichment method composed of ultra-centrifugation and detergent-based extraction was first developed. Then in-solution digestion with...
ORGANISM(S): Homo sapiens (Human) 
2016-10-07 | PXD004131 | Pride
Human Mcf7 Membrane 
ProteomicsRapid High-pH Reverse Phase StageTip for Sensitive Small-Scale Membrane Proteomic Profiling
To increase the coverage of membrane protein identification,we developed high-pH reverse phase (Hp-RP) combined with stop-and-go extraction tip (StageTip) technique, as a fast (∼15 min.). Almost 5000 proteins (∼60% membrane proteins) can be identified in only 10 μg of membrane protein digests, showi...
ORGANISM(S): Homo Sapiens (human) 
2016-09-08 | PXD004904 | JPOST Repository
ProteomicsCell membrane proteome analysis in HEK293T cells challenged with α-Synuclein amyloids
Amyloids interact with plasma membranes. Extracellular amyloids cross the plasma membrane barrier. Internalized extracellular amyloids are reported to trigger amyloidogenesis of endogenous proteins in recipient cells. To what extent these extracellular and intracellular amyloids perturb the plasma ...
ORGANISM(S): Homo sapiens (Human) 
2024-07-09 | PXD046280 | Pride
Membrane shaving Internalization Α-synuclein Plasma membrane Amyloids 
Proteomicshoneybee brain - Brain Membrane Proteome and Phosphoproteome Reveal Molecular Basis Associating with Nursing and Foraging Behaviors of Honeybee Workers
he brain is a vital organ in regulating complex social behaviors of honeybees including learning and memory. Knowledge of how brain membrane proteins and their phosphorylation underlie the age-related behavioral polyethism is still lacking. We presented the first comprehensive profiling and comparis...
ORGANISM(S): Apis mellifera (Honeybee) 
2017-09-11 | PXD006823 | Pride
Membrane Brain Honeybee Proteome Phosphoproteome 
ProteomicsMembrane complexome of Pseudomonas aeruginosa
Studies of membrane protein-protein interactions are very important to fully understand the biological function of a cell. The solubilization of proteins from the native membrane environment is a critical step in the preparation of membrane proteins that might affect the stability of protein complex...
ORGANISM(S): Escherichia coli 
2023-07-20 | PXD039702 | Pride
Membrane complexome Nanodiscs Dibma polymer 
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