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MetabolomicsProteomic analysis of osteocyte line (MLO-Y4) with knockout of cystic fibrosis transmembrane conductance regulator (CFTR) or chloride deprivation treatment

Mass spectrometry–based proteomic analysis of an osteocyte line, MLO-Y4, was conducted to profile global protein expression changes under two experimental settings, 1) CRISPR-Cas9-based knockout of CFTR versus wild-type; 2) Cl- deprivation versus normal extracellular medium.

2026-03-27 | MTBLS14165 | MetaboLights
ProteomicsCFTR interactome
The uploaded files are the RAW files used to generate the CFTR interactome dataset. The raw data were searched with ProLuCID against the human IPI database version 3.23 and search results were filtered with DTASelect 2.1.
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2015-08-13 | MSV000079235 | MassIVE
TranscriptomicsGlobal gene expression profile of small intestine from wild-type and CFTR-knockout mice.
The objective of this study was to identify changes in gene expression levels between wild-type and CFTR-knockout small intestine. CFTR-knockout mice (provided by Dr. Lane Clarke of the University of Missouri) were maintained on colyte. Keywords: gene expression comparison Four wild-type and four C...
ORGANISM(S): Mus musculus 
2010-05-25 | E-GEOD-8763 | biostudies-arrayexpress
GenomicsDifferential contribution of cis-regulatory elements to higher order chromatin structure and expression of the CFTR locus
Higher order chromatin structure establishes domains that organize the genome and coordinate gene expression. However, the molecular mechanisms controlling transcription of individual loci within a topological domain (TAD) are not fully understood. The cystic fibrosis transmembrane conductance reg...
ORGANISM(S): Homo sapiens 
2016-01-07 | E-GEOD-74709 | biostudies-arrayexpress
TranscriptomicsReduced Airway Surface pH Impairs Bacterial Killing in the Porcine Cystic Fibrosis Lung
Cystic fibrosis (CF) is a life-shortening disease caused by mutations in the cystic fibrosis transmembrane conductance regulator (CFTR) gene. Although bacterial lung infection and the resulting inflammation cause most of the morbidity and mortality, how loss of CFTR first disrupts airway host defen...
ORGANISM(S): Sus scrofa 
2012-03-29 | E-GEOD-36906 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of mouse cystic fibrosis model Cftr knockouts ileum were analysed to investigate intestinal phenotypes
Cystic fibrosis transmembrane conductance regulator (Cftr) knockout mice present the clinical features of low body weight and intestinal disease permitting an assessment of the interrelatedness of these phenotypes in a controlled environment. To identify intestinal alterations which affect body wei...
ORGANISM(S): Mus musculus 
2008-06-14 | E-GEOD-5715 | biostudies-arrayexpress
TranscriptomicsGene expression in rectal epithelia of cystic fibrosis patients
Gene expression profiles were recorded from rectal suction specimens of Cystic Fibrosis (CF) patients, carrying the CF-specific D508 mutated CFTR-allele. These profiles were compared with gene expression profiles from rectal suction specimens of non-CF subjects (control). We used Affymetrix GeneChi...
ORGANISM(S): Homo sapiens 
2013-11-28 | E-GEOD-15568 | biostudies-arrayexpress
TranscriptomicsA microRNA network regulates expression and biosynthesis of CFTR and CFTR-M-NM-^TF508.
Production of functional proteins requires multiple steps including gene transcription and post-translational processing. MicroRNAs (miRNA) can regulate individual stages of these processes. Despite the importance of the cystic fibrosis transmembrane conductance regulator (CFTR) channel for epithe...
ORGANISM(S): Homo sapiens 
2012-09-04 | E-GEOD-38956 | biostudies-arrayexpress
ProteomicsDistinct proteostasis states drive pharmacologic chaperone susceptibility for Cystic Fibrosis Transmembrane Conductance Regulator misfolding mutants
Pharmacological chaperones represent a class of therapeutic compounds for treating protein misfolding diseases. One of the most prominent examples is the FDA-approved pharmacological chaperone lumacaftor (VX-809), which has transformed cystic fibrosis (CF) therapy. CF is a fatal disease caused by mu...
ORGANISM(S): Homo sapiens (Human) 
2022-05-04 | PXD032836 | Pride
Cystic fibrosis Protein quality control Corrector Interactomics Affinity-purification mass spectrometry (ap-ms) Theratype Tandem mass tags 
TranscriptomicsTranscription profiling of pancreases of cystic fibrosis mouse with a genotype of gastrin+/-, CFTR-/- to study the gene expression patterns in cystic fibrosis.
Total RNA was prepared using TRIzol reagent from the pancreata of eight week old male mice. The genotypes were Control: gastrin+/-, CFTR+/+; and CF: gastrin+/-, CFTR-/-. All mice were on 95% black6, 5% 129Sv background. Mice were fed Peptamen from age 10 days to prevent intestinal obstruction.
ORGANISM(S): Mus musculus 
2007-12-15 | E-GEOD-769 | biostudies-arrayexpress
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