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Large-scale identification of N-linked intact glycopeptides by liquid chromatography coupled tandem mass spectrometry (LC-MS/MS) in human serum is challenging due to the wide dynamic range of serum protein abundances, the lack of a complete serum N-Glycan database and the existence of non-specifical...
ORGANISM(S): Armoracia rusticana Homo sapiens (Human) Gallus gallus (Chicken) 
2020-04-21 | PXD015622 | Pride
Cell-surface proteins represent an important class of molecules for therapeutic targeting and defining cellular phenotypes. However, their enrichment and detection via mass spectrometry-based proteomics remains challenging due to low abundance, posttranslational modifications, hydrophobic regions, a...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2024-01-21 | PXD043678 | Pride
We demonstrate the utility of lectin-magnetic nanoprobe coupled with Orbitrap HCD-CID-MS/MS for complementary glycotope-specific enrichment and site-specific glycosylation analysis of the glycoproteome. Using non-small cell lung cancer (NSCLC) cells as model, this approach allowed us to compare the ...
ORGANISM(S): Homo sapiens (Human) 
2022-02-25 | PXD010035 | Pride
This study explored the N-glycosylation profile of seven Bothrops venoms (B. alcatraz, B. cotiara, B. fonsecai, B. insularis, B. jararaca, B. jararacussu and B. moojeni).
ORGANISM(S): Bothrops sp. 
2025-11-17 | PXD057219 | Pride
The densely glycosylated spike (S) proteins that are highly exposed on the surface of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) facilitate viral attachment, entry, and membrane fusion. We have previously reported all the 22 N-glycosites and site-specific N-glycans in the S protein...
ORGANISM(S): Homo sapiens (Human) 
2021-11-03 | PXD022896 | Pride
Influenza A virus (IAV) mutates rapidly, preventing a single seasonal vaccine from targeting more than one strain, and year-to-year vaccine effectiveness is low. One challenge in designing effective vaccines is that genetic mutations frequently cause amino acid variations in IAV envelope protein hem...
ORGANISM(S): Viruses Homo sapiens (Human) Gallus gallus (Chicken) 
2020-07-03 | PXD018920 | Pride
Genomics
Clinical isolates of Myroides from Instituto Nacional de Rehabilitacion
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