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ProteomicsThe Deuterium Calculator HDX-MS Data Analysis
Hydrogen-deuterium exchange mass spectrometry (HDX-MS) is a powerful protein footprinting technique to study protein dynamics and binding; however, HDX-MS data analysis is often challenging and time consuming. Moreover, the HDX community is expanding to investigate multi-protein and highly complex p...
ORGANISM(S): Escherichia coli 
2023-01-30 | PXD036813 | Pride
Hydrogen-deuterium exchange mass spectrometry Subzero temperature uplc Hdx data analysis software Hdx-ms 
ProteomicsON-TISSUE DIRECT MONITORING OF GLOBAL HYDROGEN/DEUTERIUM EXCHANGE BY MALDI MASS SPECTROMETRY: TDXMS
Hydrogen/deuterium exchange mass spectrometric methods for protein structural analysis are conventionally performed in solution. We present Tissue Deuterium Exchange Mass Spectrometry (TDXMS), a method to directly monitor deuterium uptake on tissue, as a means to better approximate the deuterium exc...
ORGANISM(S): Rattus norvegicus (Rat) 
2016-08-15 | PXD004630 | Pride
Tissue Mass spectrometry In situ analysis H/d exchange 
ProteomicsDnaK interaction and folding of firefly luciferase studied by hydrogen/deuterium exchange – mass spectrometry
The ATP-dependent chaperones of the Hsp70 class (DnaK in E. coli) function in protein folding in cooperation with J proteins and nucleotide exchange factors (DnaJ and GrpE in E. coli, respectively). Hsp70 prevents protein aggregation, increasing the folding yield, but whether it also enhances the ra...
ORGANISM(S): Escherichia coli 
2020-01-15 | PXD016509 | Pride
Deuterium exchange Chaperone Firefly luciferase Dnak Folding 
ProteomicsHydrogen-Deuterium Exchange Reveals Catalytically Linked Protein Flexibility in Myoglobin-Mediated Intramolecular C(sp3)-H Activation
Hydrogen-Deuterium Exchange Reveals Catalytically Linked Protein Flexibility
ORGANISM(S): Escherichia coli 
2025-12-29 | PXD071026 | Pride
Myoglobin 
ProteomicsDroplet microfluidic hydrogen/deuterium exchange for investigating protein dynamics with millisecond precision exchange
Hydrogen/deuterium exchange (HDX) methods for studying protein dynamics would benefit from millisecond-scale incubations to probe intrinsically disordered proteins, highly dynamic regions and conformation changes. Here we investigate droplet microfluidics for rapid mixing to trigger D2O labelling, u...
ORGANISM(S): Bos taurus (Bovine) 
2025-06-23 | PXD063880 | Pride
Calmodulin Hdx-ms Droplet microfluidics 
ProteomicsComplementary Backbone and Side-chain Analysis of Drug – Protein Interactions: Combining Hydrogen/Deuterium Exchange and Protein Oxidative Footprinting to Discriminate the Binding of Small Molecule Therapeutics
Characterizing and distinguishing protein-ligand interactions is crucial for understanding cellular metabolism and guiding drug discovery and development. We employ hydrogen/deuterium exchange mass spectrometry (HDX-MS) and a new Fenton chemistry-based approach to protein oxidative mass spectrometry...
ORGANISM(S): Homo sapiens (Human) 
2026-03-27 | PXD050663 | Pride
Hdx Ox-ms Bcl2 Binding 
ProteomicsGroEL interaction and folding of MetF studied by hydrogen/deuterium exchange – mass spectrometry
The cylindrical chaperonin GroEL and its cofactor GroES mediate ATP-dependent protein folding in E. coli by transiently encapsulating non-native substrate in a nano-cage formed by the GroEL ring cavity and the lid-shaped GroES. We analyzed the spontaneous and chaperonin-assisted folding of the essen...
ORGANISM(S): Escherichia coli 
2020-03-05 | PXD016666 | Pride
Groel Chaperone Folding Metf Hydrogen/deuterium exchange Chaperonin 
ProteomicsStabilization of diverse amyloidogenic antibody light chains by small molecules revealed by hydrogen-deuterium exchange mass spectrometry
Antibody light chains can aggregate as amyloid fibrils to cause systemic AL amyloidosis. Amyloid deposition requires unfolding of the light chain from its native state, but the mechanistic details of how this occurs are not fully understood. Inhibiting amyloid formation by stabilizing the precursor ...
ORGANISM(S): Homo sapiens (Human) 
2026-07-27 | PXD068745 | Pride
ProteomicsDetermination of highly accurate small molecule:protein affinity constants at amino acid resolution using Hydrogen Deuterium Exchange Mass Spectrometry (HDX-MS)
Hydrogen Deuterium Exchange Mass Spectrometry (HDX-MS) has been widely adopted as part of the drug development pipeline, frequently used in both the development of biopharmaceuticals and small molecule compound development to assess target engagement spatially. Frequently, HDX-MS analyses are conduc...
ORGANISM(S): Plasmodium falciparum 58.1 
2026-04-01 | PXD070051 | Pride
ProteomicsSimple and Fast Maximally Deuterated Control (maxD) preparation for HDX MS Experiments
During the analysis steps of hydrogen deuterium exchange (HDX) mass spectrometry (MS) there is an unavoidable loss of deuterons, or back-exchange. Understanding back-exchange is necessary to correct for loss during analysis, to calculate the absolute amount of exchange, and to ensure that deuterium ...
ORGANISM(S): Bos taurus (Bovine) Homo sapiens (Human) Equus caballus (Horse) 
2022-07-08 | PXD032924 | Pride
Hdx ms Mass spectrometry Hydrogen deuterium exchange Back-exchange Maximally deuterated samples 
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