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ProteomicsRobust dimethyl-based multiplex-DIA doubles single-cell proteome depth via a reference channel
Single-cell proteomics aims to characterize biological function and heterogeneity at the level of proteins in an unbiased manner. It is currently limited in proteomic depth, throughput, and robustness, which we address here by a streamlined multiplexed workflow using data-independent acquisition (mD...
ORGANISM(S): Homo sapiens (Human) 
2023-08-30 | PXD038632 | Pride
Mass spectrometry Dimethyl labeling Multiplexing Dia Single cells 
ProteomicsHST-MRM-MS: A Novel High-Sample-Throughput Multiple Reaction Monitoring Mass Spectrometric Method for Multiplex Absolute Quantitation of Hepatocellular Carcinoma Serum Biomarker
Absolute quantification of clinical biomarkers by mass spectrometry (MS) has been challenged due to low sample-throughput of current multiple reaction monitoring (MRM) methods. For this problem to be overcome, in this work, a novel high-sample-throughput multiple reaction monitoring mass spectrometr...
ORGANISM(S): Homo Sapiens 
2020-02-23 | PXD040708 |
ProteomicsRobust dimethyl-based multiplex-DIA doubles single-cell proteome depth via a reference channel
Single-cell proteomics aims to characterize biological function and heterogeneity at the level of proteins in an unbiased manner. It is currently limited in proteomic depth, throughput, and robustness, which we address here by a streamlined multiplexed workflow using data-independent acquisition (mD...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2022-12-22 | MSV000090956 | MassIVE
UnknownA novel boronic acid-based fluorescent sensor for the selective detection of l-lysine in food samples and cells.
Not available
S-EPMC11969143 | biostudies-literature
ProteomicsTowards understanding male infertility after spinal cord injury through quantitative proteomics
The study of male infertility after spinal cord injury (SCI) has enhanced the understanding of seminal plasma (SP) as an important regulator of spermatozoa function. However, the most important factors leading to the diminished sperm motility and viability observed in semen of men with SCI remained ...
ORGANISM(S): Homo sapiens (Human) 
2016-02-01 | PXD002145 | Pride
Anejaculation Human seminal plasma Spinal cord injury Male infertility Quantitative proteomics Lc-ms/ms 
UnknownStructural origins for the product specificity of SET domain protein methyltransferases.
Not available
S-EPMC2634886 | biostudies-literature
ProteomicsProbing tyrosine kinase inhibitors specificity
Using a three-pronged MS-based proteomic approach, we assessed the mode-of-action of four clinically-relevant tyrosine kinase inhibitors in an epithelial cancer cell line. Here we defined the: (I) semi-quantitative tyrosine kinome; (II) drug interactome; and (III) drug-induced effects on the tyrosin...
ORGANISM(S): Homo sapiens (Human) 
2014-05-12 | PXD000681 | Pride
Human Tyrosine kinase inhibitors Chemical proteomics Phosphoproteomics Phosphotyrosine Lc-ms/ms 
ProteomicsLysargiNase cleavage specificity characterization
Determination of LysargiNase and tryptic ckeavage efficiency at dimethylated Lys residues
ORGANISM(S): Escherichia coli 
2014-12-16 | PXD001379 | Pride
E.coli Dimethyl-lys Lysarginase Trypsin 
UnknownPoly[[tris-(μ-4,4'-bipyridine-κ(2)N:N')bis-(μ-l-lysinato-κ(3)N(1),O(1):O(1'))dizinc(II)] tetra-nitrate 0.6-hydrate dimethyl-formamide disolvate].
Not available
S-EPMC3344362 | biostudies-literature
ProteomicsTerminal alkynes react with active-site cysteine nucleophiles
Propargyl labeled ubiquitin was immobilized on beads and used for click-chemistry based pull downs. Covalently linked proteins were washed and digested off-bead, or eluted with strong acid and loaded into a SDS-PAGE gel. Digestion with Lys-C, trypsin consequetively and LC-MS/MS. Both dimethyl labeli...
ORGANISM(S): Mus musculus (Mouse) 
2013-08-14 | PXD000073 | Pride
Propargyl Mouse Click chemistry Ubiquitin Deubiquitinating enzymes Dub 
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