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ProteomicsEvaluation of N8-propargyl spermidine and spermine as substrates of deoxyhypusine synthase in the in vitro hypusination of eIF5A and RIPK1
eIF5A is a well-established protein substrate of deoxyhypusine synthase (DHS), which also requires spermidine as a small-molecule substrate. At the end of 2024, a publication claimed that RIPK1 is an additional DHS substrate and that N8-propargyl spermidine can also serve as a DHS substrate. To eval...
ORGANISM(S): Homo sapiens (Human) 
2026-03-13 | PXD071577 | Pride
N8-propargyl spermidine Dhs Eif5a Chemical probe Hypusination Deoxyhypusine synthase Ripk1 
Genomics
clodinafop-propargyl-degrading
PRJNA1067419 | ENA
ProteomicsEvaluation of N8-propargyl spermidine as a substrate of deoxyhypusine synthase in cellulo
Two complementary experiments were performed to evaluate whether N8-propargyl spermidine is a substrate of deoxyhypusine synthase in cells. HEK293 and mouse embryonic fibroblast (MEF) cells were incubated with either N8-propargyl spermidine or spermidine, lysed, and subjected to CuAAC ligation with ...
ORGANISM(S): Mus musculus (Mouse) Homo sapiens (Human) 
2026-03-13 | PXD071477 | Pride
Chemical probe Hypusination Hek293 N8-propargyl spermidine Mef 
ProteomicsN8-propargyl spermidine as a chemical probe for sensing of ALDH1A1 activity in cells
A549 cells were incubated with N8-propargyl spermidine, N1-propargyl spermidine, or native spermidine. Protein conjugates formed in cellulo were analyzed as follows. After cell lysis, probe-protein adducts were subjected to bioorthogonal CuAAC ligation and affinity enrichment on NeutrAvidin beads. T...
ORGANISM(S): Homo sapiens (Human) 
2026-05-28 | PXD070993 | Pride
Chemical probe Aldh1a1 A549 Activity-based probe N8-propargyl spermidine 
OtherEnzymatic or in vivo installation of propargyl groups in combination with click chemistry enables enrichment and detection of methyltransferase target sites in RNA
m6A is the most abundant internal modification in eukaryotic mRNA. It is introduced by METTL3-METTL14 and tunes mRNA metabolism, impacting cell differentiation and development. Precise transcriptome-wide assignment of m6A sites is of utmost importance. However, m6A does not interfere with Watson-Cri...
ORGANISM(S): Homo sapiens synthetic construct 
2018-02-21 | GSE109298 | GEO
GenomicsEnzymatic or in vivo installation of propargyl groups in combination with click chemistry enables enrichment and detection of methyltransferase target sites in RNA
Enzymatic or in vivo installation of propargyl groups in combination with click chemistry enables enrichment and detection of methyltransferase target sites in RNA
PRJNA430372 | ENA
ProteomicsEvaluation of N8-propargyl spermidine (Probe 1) as a substrate of deoxyhypusine synthase in cellulo
Two complementary experiments were performed to evaluate whether N8-propargyl spermidine is a substrate of deoxyhypusine synthase in cells. HEK293 and mouse embryonic fibroblast (MEF) cells were incubated with either N8-propargyl spermidine (Probe 1) or spermidine, lysed, and subjected to CuAAC liga...
ORGANISM(S): Mus musculus (Mouse) Homo sapiens (Human) 
2026-06-23 | PXD080059 | Pride
N8-propargyl spermidine Mef Chemical probe Hypusination Hek293 
ProteomicsChemical proteomics reveals protein tyrosination extends beyond the alpha-tubulins in human cells.
Tubulin detyrosination-tyrosination cycle regulates the stability of microtubules. Thus far described on α-tubulins, the tyrosination level is maintained by a single tubulin-tyrosine ligase (TTL). However, the precise dynamics and tubulin isoforms which undergo (de)tyrosination in neurons are unknow...
ORGANISM(S): Homo sapiens (Human) 
2022-10-20 | PXD037402 | Pride
Ingns Dia-nn Tyrosination Sp2e Sh-sy5y 
UnknownCopper-Catalyzed Decarboxylative Trifluoromethylation of Propargyl Bromodifluoroacetates.
Not available
S-EPMC4185158 | biostudies-literature
UnknownPhotoredox cobalt-catalyzed regio-, diastereo- and enantioselective propargylation of aldehydes via propargyl radicals.
Not available
S-EPMC10415309 | biostudies-literature
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