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MetabolomicsP53 dependent hypusination of eIF5A affect mitochondrial translation and senescence immune surveillance
Cellular senescence is characterized by a permanent growth arrest and is associated with tissue aging and cancer. Senescent cells secrete a number of different cytokines referred to as the senescence-associated secretory phenotype (SASP), which impacts the surrounding tissue and immune response. Her...
2024-08-14 | MTBLS10690 | MetaboLights
TranscriptomicsTranscription profiling of S. cerevisiae temperature sensitive eIF5A mutant shows accumulation of transcripts targeted to the Nonsense Mediated Decay pathway
The highly conserved protein eIF5A found in archaea and all eucaryotes uniquely contains the posttranslationally formed amino acid hypusine. Despite being essential the functions of this protein and its modification remain unclear. To gain more insight into these functions temperature sensitive muta...
ORGANISM(S): Saccharomyces cerevisiae 
2008-06-13 | E-GEOD-5290 | biostudies-arrayexpress
ProteomicsInteraction of human eIF5A and DHS K329A variant studied by HDX-MS
Hypusination is a unique post-translational modification of the eukaryotic translation factor 5A (eIF5A) that is essential for overcoming ribosome stalling at polyproline sequence stretches. The initial step of hypusination, the formation of deoxyhypusine, is catalyzed by deoxyhypusine synthase (DHS...
ORGANISM(S): Homo sapiens (Human) Escherichia coli 
2023-05-10 | PXD040460 | Pride
Eif5a Hdx-ms Synapt-g2 si Dhs k329a variant 
ProteomicsBiochemical profile of the A.thaliana hypusination pathway
The eukaryotic translation elongation factor eIF5A is the only protein known to contain the unusual amino acid hypusine which is essential for its biological activity. This posttranslational modification is achieved by the sequential action of the enzymes deoxyhypusine synthase and deoxyhypusine hyd...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2016-07-09 | PXD000880 | Pride
Spermidine Eif5a Abscisic acid 2d-electrophoresis Hypusine 
ProteomicsEssential role of translation factor eIF5a in cytokine and cell cycle regulation in primary mouse CD8 T lymphocyte
Translation of specific mRNAs is tightly regulated in primary T cells to ensure correct timing and precise delivery of protein syntheses in response to environmental cues. Translation factor eIF5a is known to be important for protein synthesis and is expected to regulate translation of certain diffi...
ORGANISM(S): Mus musculus (Mouse) 
2022-11-11 | PXD021063 | Pride
Cytokine Lymphocyte Hypusine Proliferation Eif5a 
ProteomicsEIF5A couples translational control with transcriptional reprogramming through chromocenter reorganization during spermiogenesis
Translation initiation factor eIF5A facilitates protein synthesis and impacts diverse biological processes, yet its role in transcriptional regulation is poorly understood. Here we find eIF5A highly expressed in diverse spermatogenic cell types. Conditional knockout of Eif5a (SKO) causes complete in...
ORGANISM(S): Mus Musculus 
PXD067244 |
MetabolomicsHypusination Orchestrates the Antimicrobial Response of Macrophages
Innate responses of myeloid cells defend against pathogenic bacteria via inducible effectors. Deoxyhypusine synthase (DHPS) catalyzes the transfer of the N-moiety of spermidine to the lysine-50 residue of eukaryotic translation initiation factor 5A (EIF5A) to form the amino acid hypusine. Hypusinate...
2021-04-06 | MTBLS607 | MetaboLights
ProteomicsEvaluation of N8-propargyl spermidine and spermine as substrates of deoxyhypusine synthase in the in vitro hypusination of eIF5A and RIPK1
eIF5A is a well-established protein substrate of deoxyhypusine synthase (DHS), which also requires spermidine as a small-molecule substrate. At the end of 2024, a publication claimed that RIPK1 is an additional DHS substrate and that N8-propargyl spermidine can also serve as a DHS substrate. To eval...
ORGANISM(S): Homo sapiens (Human) 
2026-03-13 | PXD071577 | Pride
N8-propargyl spermidine Dhs Eif5a Chemical probe Hypusination Deoxyhypusine synthase Ripk1 
ProteomicsPolyamines and eIF5A hypusination modulate mitochondrial respiration and macrophage activation
How cells adapt metabolism to meet demands is an active area of interest across biology. Amongst a broad range of functions, the polyamine spermidine is needed to hypusinate the translation factor eukaryotic initiation factor 5A (eIF5A). We show here that hypusinated eIF5A (eIF5AH) promotes the effi...
ORGANISM(S): Mus musculus (Mouse) 
2019-05-24 | PXD013443 | Pride
Proteomics Macrophage Polyamine Eif5a 
ProteomicsEIF5A hypusinaion in hepatic cells
determin the impact fatty acids and spermidine on EIF5A hysusinaion, and examined the role of EIF5A hysusination in hepatocytes
ORGANISM(S): Mus musculus (Mouse) 
2022-09-21 | PXD028714 | Pride
Mouse Hepatic cells 
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