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ProteomicsTransforming chemical proteomics into high-throughput method using SP2E workflow.
Protein post-translational modifications (PTMs) play a critical role in regulation of protein catalytic activity, localization and protein-protein interactions. An attachment of PTMs onto proteins significantly diversify their structure and function resulting in so called proteoforms. However, the s...
ORGANISM(S): Homo sapiens (Human) 
2022-07-04 | PXD033644 | Pride
Method development Ampylation 
UnknownTransforming Chemical Proteomics Enrichment into a High-Throughput Method Using an SP2E Workflow.
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S-EPMC9326820 | biostudies-literature
ProteomicsChemical proteomics reveal the inventory of pyrroloquinoline quinone binding proteins in bacteria
Pyrroloquinoline quinone (PQQ) is a bacterial redox cofactor enabling enzyme catalysis in various sugar and alcohol dehydrogenases. However, its proposed additional role as a "longevity vitamin" lacks a clear molecular basis and is thus highly debated. Here, we applied chemical proteomics to identif...
ORGANISM(S): Homo sapiens (Human) Escherichia coli Pseudomonas putida KT2440 
2026-04-27 | PXD069310 | Pride
Covalent binding Chemcial proteomics Pyrroloquinoline quinone 
ProteomicsChemical Proteomics Enhances the Understanding of 2AA Stress in Salmonella enterica
Pyridoxal 5’phosphate (PLP) is an essential cofactor for enzymes that catalyze diverse reactions in central metabolism. 2-aminoacrylate (2AA) is a reactive enamine and an obligate catalytic intermediate in some PLP-mediated reactions. In the absence of the enamine/imine deaminase RidA, Salmonella en...
ORGANISM(S): Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) 
2025-05-24 | PXD060390 | Pride
2-aminoacrylate Pyridoxal-phosphate-dependent enzymes Plp Chemical proteomics Rida 
ProteomicsScreening privileged alkylguanidinium motifs under host-mimicking conditions reveals a novel antibiotic with an unconventional mode of action
Screening large molecule libraries against pathogenic bacteria is often challenged by a low hit rate due to limited uptake, underrepresentation of antibiotic structural motifs and assays which do not resemble the infection conditions. To address these limitations, we here screen a library focused on...
ORGANISM(S): Staphylococcus aureus 
2024-07-17 | PXD051986 | Pride
Antibiotics Chemical proteomics Sar Moa studies Guanidinium 
ProteomicsChemical proteomics reveals protein tyrosination extends beyond the alpha-tubulins in human cells.
Tubulin detyrosination-tyrosination cycle regulates the stability of microtubules. Thus far described on α-tubulins, the tyrosination level is maintained by a single tubulin-tyrosine ligase (TTL). However, the precise dynamics and tubulin isoforms which undergo (de)tyrosination in neurons are unknow...
ORGANISM(S): Homo sapiens (Human) 
2022-10-20 | PXD037402 | Pride
Ingns Dia-nn Tyrosination Sp2e Sh-sy5y 
ProteomicsDeazaflavin-Diazirine Energy-Transfer (DarT) Photoproximity Labelling of the HER2 Interactome Using Antibody Conjugates
Elucidating protein-protein interactions plays a crucial part in understanding disease mecha-nisms and advancing pharmacological research. Photocatalytic proximity labelling using anti-body–catalyst conjugates enables the highly target-specific analysis of protein-protein inter-actions on the cell s...
ORGANISM(S): Homo sapiens (Human) 
2026-08-04 | PXD076830 | Pride
ProteomicsEnergy transfer photoproximity labelling in live cells using an organic cofactor
Photocatalytic proximity labelling has recently emerged as a powerful tool to resolve a wide variety of biomolecular and cellular interactions. While the use of high-resolution probe species, such as diazirines, enables cell-surface protein labelling with nanometre precision by generating highly rea...
ORGANISM(S): Homo sapiens (Human) 
2025-12-08 | PXD056778 | Pride
Photoproximity Diazirine Lfq Dart 
ProteomicsUnsaturated phosphorus electrophiles to probe protein tyrosine phosphatases
Protein tyrosine phosphatases (PTPs) represent an important pharmacological target. To this end, broad spectrum electrophilic, phosphotyrosine-mimicking probes have been developed to covalently capture the catalytic site of these enzymes. Despite these efforts, there is still a high demand for synth...
ORGANISM(S): Homo sapiens (Human) 
2026-03-03 | PXD068999 | Pride
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