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ProteomicsCross-linking mass spectrometry of purified human endosomes (DHSO, DMTMM)
EndoMAP aims to systematically dissect the human endosomal protein complexes. DHSO/DMTMM cross-linking experiment was performed on freshly prepared endosomal pellets from HEK293 cells to capture protein interactions and structures in purified organelles.
ORGANISM(S): Homo sapiens (Human) 
2025-03-29 | PXD059547 | Pride
Dhso Cross-linking Human Dmtmm Endosomes Endomap 
UnknownFluorescence Quenching of Xanthene Dyes during Amide Bond Formation Using DMTMM.
Not available
S-EPMC9494652 | biostudies-literature
UnknownDMTMM-Mediated Intramolecular Cyclization of Acidic Residues in Peptides/Proteins.
Not available
S-EPMC7905807 | biostudies-literature
ProteomicsTissue Transglutaminase 2 has higher affinity for relaxed than for stretched fibronectin fibers
Chemical cross-linking coupled to mass spectrometry was used to study the interaction between fibronectin (Fn; full-length or gelatin-binding domain fragment = Fn 45) with tissue transglutaminase 2 in oxidized and non-oxidized form. The complexes were cross-linked with disuccinimidyl suberate (DSS) ...
ORGANISM(S): Homo sapiens (Human) 
2023-12-20 | PXD043976 | Pride
Chemical cross-linking  dss  pdh  dmtmm  structural proteomics  fibronectin Tissue transglutaminase 2 
ProteomicsExpanding the cross-link coverage of a dihydrazide-based carboxyl-group specific chemical cross-linking strategy - Dataset 1: Model proteins
The conditions for chemical protein-protein cross-linking using the reagents pimelic dihydrazide (PDH) and 4-(4,6-Dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium (DMTMM) chloride were optimized using a set of five model proteins and two large complexes, the 20S proteasome and the 70S ribosome. Th...
ORGANISM(S): Bos taurus (Bovine) Oryctolagus cuniculus (Rabbit) Gallus gallus (Chicken) 
2021-01-04 | PXD019724 | Pride
Chemical cross-linking  pdh  dmtmm  model proteins  method optimization 
ProteomicsExpanding the cross-link coverage of a dihydrazide-based carboxyl-group specific chemical cross-linking strategy - Dataset 2: Bovine 20S proteasome
The conditions for chemical protein-protein cross-linking using the reagents pimelic dihydrazide (PDH) and 4-(4,6-Dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium (DMTMM) chloride were optimized using a set of five model proteins and two large complexes, the 20S proteasome and the 70S ribosome. Th...
ORGANISM(S): Bos taurus (Bovine) 
2021-01-04 | PXD019507 | Pride
Chemical cross-linking  pdh  dmtmm  20s proteasome  method optimization 
ProteomicsExpanding the cross-link coverage of a dihydrazide-based carboxyl-group specific chemical cross-linking strategy - Dataset 3: E. coli 70S ribosome
The conditions for chemical protein-protein cross-linking using the reagents pimelic dihydrazide (PDH) and 4-(4,6-Dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium (DMTMM) chloride were optimized using a set of five model proteins and two large complexes, the 20S proteasome and the 70S ribosome. Th...
ORGANISM(S): Escherichia coli 
2021-01-04 | PXD019508 | Pride
Chemical cross-linking  pdh  dmtmm  70s ribosome  method optimization 
ProteomicsIdentification of a direct interaction between the Fab domains of IgG antibodies and human FcRn upon IgG-FcRn complex formation
Chemical cross-linking coupled to mass spectrometry was used to study the interaction between IgG-type antibodies and the neonatal Fc receptor (FcRn). Different cross-linking chemistries were used, including disuccinimidyl suberate (DSS); a combination of pimelic dihydrazide (PDH) in combination wit...
ORGANISM(S): Homo sapiens (Human) 
2024-12-03 | PXD044405 | Pride
Chemical cross-linking  dss  pdh  dmtmm  xplex  structural proteomics  antibody – receptor interactions 
ProteomicsSaturation mutagenesis of alpha-synuclein reveals monomer fold that modulates aggregation
LUM2_890611, 890654, 890655, 890656, 890657: 5 replicates; WT alpha-synuclein crosslinked with DMTMM for 10 min. LUM2_890612, 890658, 890659, 890660, 890661: 5 replicates; A53T alpha-synuclein crosslinked with DMTMM for 10 min. LUM2_954549-954553: 5 replicates; E13K alpha-synuclein crosslinked with ...
ORGANISM(S): Escherichia Coli (ncbitaxon:562) Homo Sapiens (ncbitaxon:9606) 
2023-05-16 | MSV000091963 | MassIVE
ProteomicsA precisely positioned MED12 activation helix stimulates CDK8 kinase activity
Chemical cross-linking coupled to mass spectrometry was used to study binary and ternary complexes involving cyclin-dependent kinase 8 (CDK8), cyclin-C, and subunit 12 of the Mediator complex (MED12). Cross-linking was performed using different cross-linking chemistries: (1) disuccinimidyl suberate ...
ORGANISM(S): Homo sapiens (Human) 
2020-01-16 | PXD015394 | Pride
Chemical cross-linking  dss  pdh  dmtmm  structural proteomics  mediator complex  kinase 
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