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ProteomicsCross-linking mass spectrometry of purified human endosomes
EndoMAP aims to systematically dissect the human endosomal protein complexes. Cross-linking experiments were performed on two biologically independent replicates of freshly prepared endosomal pellets from HEK293 cells to capture protein interactions and structures in purified organelles.
ORGANISM(S): Homo sapiens (Human) 
2025-03-29 | PXD054728 | Pride
Cross-linking Human Endosomes Dsso Endomap 
ProteomicsCross-linking mass spectrometry of purified human endosomes (DHSO, DMTMM)
EndoMAP aims to systematically dissect the human endosomal protein complexes. DHSO/DMTMM cross-linking experiment was performed on freshly prepared endosomal pellets from HEK293 cells to capture protein interactions and structures in purified organelles.
ORGANISM(S): Homo sapiens (Human) 
2025-03-29 | PXD059547 | Pride
Dhso Cross-linking Human Dmtmm Endosomes Endomap 
ProteomicsBlue Native PAGE co-fractionation of purified human endosomes
EndoMAP aims to systematically dissect the human endosomal protein complexes. To purify endosomes from human cells, a modified Endo-IP protocol was developed to be compatible with complexomics methodologies and evaluated by DIA-MS. Three independent biological replicates of human endosomes purified ...
ORGANISM(S): Homo sapiens (Human) 
2025-03-29 | PXD054684 | Pride
Co-fractionation Human Endosomes Bn-page Endomap 
ProteomicsCharacterization of endosomes and interactions from TMEM230 and TMEM9/9B mutants in human neurons
EndoMAP aims to systematically dissect the human endosomal protein complexes. To examine TMEM9 and TMEM230 interactions, we generated TMEM230 and TMEM9 knockouts (KOs) using CRISPR/Cas9 in human embryonic stem (ES) cells and converted the cells to induced neurons (iNeurons). TMEM230 and TMEM9 were i...
ORGANISM(S): Homo sapiens (Human) 
2025-03-29 | PXD054765 | Pride
Tmt Human Tmem9 Endosomes Ineurons Tmem230 Endomap 
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