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ProteomicsElectrophile scanning by chemical prote-omics reveals a potent pan-active DUB probe for investigation of deubiquitinase activity in live cells
Deubiquitinases (DUBs) are proteases that hydrolyze isopeptide bonds linking ubiquitin to protein substrates, which can lead to reduced substrate degradation through the ubiquitin proteasome system. Deregulation of DUB activity has been im-plicated in many disease states, including cancer, neurodege...
ORGANISM(S): Homo sapiens (Human) 
2024-01-03 | PXD035417 | Pride
Ubiquitin-activity base probe Deubiquitinase Chemical probe Activity-based probe Electrophilic war-head Ubiquitin Dub Enzyme targeting Myc-deregulated cancer 
ProteomicsDeubiquitinase Vulnerabilities Identified through Activity Based Protein Profiling in Non-Small Cell Lung Cancer
To aid in the prioritization of deubiquitinases (DUBs) as anticancer targets, we developed an approach combining activity-based protein profiling (ABPP) with mass spectrometry in both non-small cell lung cancer (NSCLC) tumor tissues and cell lines along with analysis of available RNA interference an...
ORGANISM(S): Homo sapiens (Human) 
2022-07-05 | PXD028802 | Pride
Human Activity-based protein profiling Deubiquitinase Lung cancer 
ProteomicsDevelopment of Substrate-Directed Activity-Based Probes via Disulfide-Trapping for Site-Specific Interrogation of Nucleosomal Deubiquitination
Activity-based ubiquitin probes (Ub-ABPs) have been instrumental in interrogating deubiquitinases (DUBs), yet conventional Ub-ABPs lack the substrate-specific and site-specific contextual cues required to investigate nucleosomal DUBs, whose activity is tightly regulated by the chromatin environments...
ORGANISM(S): Homo Sapiens 
2025-12-30 | PXD072417 |
ProteomicsA linear diubiquitin-based probe for efficient and selective detection of the deubiquitinating enzyme OTULIN
The methionine1 (M1)-specific deubiquitinase OTULIN acts as a negative regulator of NF-ΚB signaling and immune homeostasis. By replacing G76 in distal ubiquitin by dehydroalanine we designed the diubiquitin (diUb) probe UbG76Dha-Ub (OTULIN ABP) that couples to the catalytic site of OTULIN and thereb...
ORGANISM(S): Homo sapiens (Human) 
2017-09-19 | PXD006868 | Pride
Human Otulin 
ProteomicsDiscovery of highly Potent and Selective Activity-Based Probes for the Deubiquitylating Enzyme USP30
Ubiquitin-specific protease 30 (USP30) is a deubiquitylating enzyme (DUB) localized in the mitochondrial membrane, which is related to PINK1/Parkin-mediated mitophagy, pexophagy, BAX/BAK-dependent apoptosis, and IKKβ-USP30-ACLY-regulated lipogenesis/tumorigenesis. Mission therapeutics pointed their ...
ORGANISM(S): Homo sapiens (Human) 
2024-05-22 | PXD044792 | Pride
Usp30  activity-based probes  chemical probes  desi  deubiquitinase. 
ProteomicsChemical tools to define and manipulate interferon-induced Ubl protease USP18
Ubiquitin-specific protease 18 (USP18) is a multifunctional cysteine protease primarily responsible for deconjugating interferon-induced ubiquitin-like (Ubl) modifier ISG15 from protein substrates. Here, we report the design and synthesis of activity-based probes (ABPs) capable of selectively detect...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2024-03-26 | MSV000094406 | MassIVE
ProteomicsIdentification of deubiquitinases controlled by extracellular matrix stiffness in cancer cells
The project was aimed at identifying deubiquitinases (DUBs) involved in cancer cell mechanosignaling. Lysates from cancer cells grown on collagen matrices with varying stiffnesses were incubated with an activity-based HA-tagged ubiquitin probe that covalently binds DUB active site. To identify DUBs ...
ORGANISM(S): Homo sapiens (Human) 
2025-08-22 | PXD054010 | Pride
Lc-ms/ms Immunopurification Dub abp Ecm stiffness 
UnknownA Linear Diubiquitin-Based Probe for Efficient and Selective Detection of the Deubiquitinating Enzyme OTULIN.
Not available
S-EPMC5658516 | biostudies-literature
ProteomicsDUB Profiling of 20 different breast cancer cell lines.
De-ubiquitinating enzymess from 20 Different breast cancer cell lines were purified using an Affinity Binding Probe.
ORGANISM(S): Homo sapiens (Human) 
2020-01-29 | PXD013161 | Pride
Tgfb Breast cancer Ubiquitin De-ubiquitinase 
UnknownInternal Ubiquitin Electrophiles for Covalent Trapping and Inhibition of Deubiquitinases.
Not available
S-EPMC12631012 | biostudies-literature
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