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Here, we present a simple and robust N-linked glycoproteome enrichment protocol optimized for deep coverage of the plasma membrane proteome. The procedure was applied to characterize the cell surface proteome of 15 standard laboratory human cell lines and three primary cell types. We observed signif...
ORGANISM(S): Homo sapiens (Human) 
2017-03-27 | PXD005846 | Pride
Quorum sensing in Burkholderia cenocepacia H111 involves two signalling systems that depend on different signal molecules, namely N-acyl homoserine lactones (AHLs) and the diffusible signal factor cis-2-dodecenoic acid (BDSF). Previous studies have shown that AHLs and BDSF control similar phenotypic...
ORGANISM(S): Burkholderia cenocepacia H111 
Data from ProteomeXchange, PXD ID: PXD001695. File: 20140827_EXQ00_FaHo_SA_SWI3_03.mzml. Published as part of Mol Cell Proteomics. 2015 Apr 17 . From the Abstract: {{i}} ... Here we develop a high-throughput workflow capable of dealing with large numbers of low or medium complexity samples and spec...
ORGANISM(S): Yeast 
Chemoproteomics relies on precise and comprehensive quantification of protein–compound interactions, yet methodological comparisons across mass spectrometry platforms remain scarce. Here, we systematically benchmark tandem mass tag data-dependent acquisition (TMT-DDA) on an Orbitrap against label-fr...
ORGANISM(S): Homo sapiens (Human) 
2026-09-21 | PXD065032 | Pride
Using an integrated systems approach, the expressed proteome of B. diazoefficiens strain 110scp4 was measured under i) normal, oxic growth, and ii) microoxic growth condtions. This included, as a first step, the sequencing and de novo assembly of the genome of this widely used rhizobial model strain...
ORGANISM(S): Bradyrhizobium diazoefficiens 
2019-05-30 | PXD012491 | Pride
B. cenocepacia is an opportunistic human pathogen that is particularly problematic for patients suffering from cystic fibrosis (CF). In the CF lung, bacteria grow to high densities within the viscous mucus that is limited in oxygen. Pseudomonas aeruginosa, the dominant pathogen in CF patients, is kn...
ORGANISM(S): Burkholderia cenocepacia H111 
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