Sort   by:  
 Page size 
ProteomicsMechanisms of site-specific methylation by a highly conserved elongation factor 1A lysine methyltransferase
Translation elongation factor 1A (eEF1A) is an essential and highly conserved protein required for protein synthesis in eukaryotes. In both Saccharomyces cerevisiae and human, five different methyltransferase enzymes methylate specific residues on eEF1A, making eEF1A the eukaryotic protein targeted ...
ORGANISM(S): Homo sapiens (Human) Saccharomyces cerevisiae (Baker's yeast) 
2024-01-03 | PXD042599 | Pride
Crosslinking mass spectrometry Protein methyltransferase Protein methylation 
ProteomicsMETTL13 modulates mRNA translation through methylation of eEF1A at Lys55 and the N-terminus
The experiment aimed at identifying lysine methylation dependent interactions for eEF1A. FLAG-tagged wild type eEF1A and a methylation deficient mutant, carrying lysine-to-arginine mutations of the well-established methylation sites (Lys36, Lys55, Lys79, Lys165 and Lys318) were overexpressed in HEK...
ORGANISM(S): Homo sapiens (Human) 
2018-07-04 | PXD009895 | Pride
Methylation Eef1a Interactome 
ProteomicsMETTL13 modulates mRNA translation through methylation of eEF1A at Lys55 and the N-terminus, part 2
The experiment aimed at characterizing the methylation status of eEF1A in cells (HeLa) stressed using a panel of drugs including anisomycin, cycloheximide, 4NQO and adenosine dialdehyde (AdOx).
ORGANISM(S): Homo sapiens (Human) 
2018-07-04 | PXD009914 | Pride
Methylation Ptm Eef1a 
ProteomicsMS data for Chp1 is a dedicated chaperone at the ribosome that safeguards eEF1A biogenesis
Cotranslational protein folding depends on general chaperones that engage highly diverse nascent chains at the ribosomes. It is not clear how this promiscuous mechanism ensure efficient production of challenging clients such as highly expressed proteins. Here we find that the biogenesis of the abund...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2024-06-16 | PXD043391 | Pride
Ribosome-associated chaperone Hsf1 activation Eef1a biogenesis 
TranscriptomicsTranscription profiling of rat AFTER IN vivo administration OF Gamendazole AND Lonidamine
rat testis time changes; Experimental details are found at Tash et al, Gamendazole, an orally active indazole carboxylic acid male contraceptive agent, targets HSP-90, eEF1A, and stimulates IL-1 transcription in Sertoli cells, Biol. Reprod. (2007) under review Experiment Overall Design: Adult male r...
ORGANISM(S): Rattus rattus 
2008-04-10 | E-GEOD-8485 | biostudies-arrayexpress
GenomicsDedicated chaperone at the ribosome safeguards the proteostasis network during eEF1A biogenesis
Dedicated chaperone at the ribosome safeguards the proteostasis network during eEF1A biogenesis
PRJNA915314 | ENA
ProteomicseEF1A peptide pull-down LC-MS/MS
Peptide pull-down using synthetic peptides corresponding to unmodified and methylated human eEF1A.
ORGANISM(S): Homo sapiens (Human) 
2018-07-04 | PXD008115 | Pride
Methylation Ptm Lc-ms/ms 
UnknowneEF1A: thinking outside the ribosome.
Not available
S-EPMC2898402 | biostudies-literature
TranscriptomicsExpression data from Saccharomyces cerevisiae with eEF1A overexpression
The canonical role of eEF1A is to deliver the aminoacyl tRNA to the ribosome, we have used the yeast model system to investigate further roles for this protein. We used microarray to study the transcriptomic effects of elevated levels of eEF1A on yeast cells during log phase growth
ORGANISM(S): Saccharomyces cerevisiae Schizosaccharomyces pombe 
2016-10-29 | GSE89286 | GEO
TranscriptomicsDedicated chaperone at the ribosome safeguards the proteostasis network during eEF1A biogenesis
Cotranslational protein folding depends on general chaperones that engage highly diverse nascent chains at the ribosomes. Here we find that the universal cotranslational machinery adapts to accommodate the challenging biogenesis of abundantly expressed eukaryotic translation elongation factor 1A (eE...
ORGANISM(S): Saccharomyces cerevisiae 
2024-01-16 | GSE221651 | GEO
Sort   by:  
 Page size