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TranscriptomicsRocaglates convert DEAD-box protein eIF4A into a sequence-selective translational repressor
Rocaglamide A (RocA) typifies a class of protein synthesis inhibitors that selectively kill aneuploid tumor cells and repress translation of specific mRNAs. RocA targets eukaryotic initiation factor 4A (eIF4A), an ATP-dependent DEAD-box RNA helicase; its mRNA selectivity is proposed to reflect highl...
ORGANISM(S): synthetic construct 
2016-06-15 | E-GEOD-79392 | biostudies-arrayexpress
TranscriptomicsRocaglamide A converts RNA helicase eIF4A into a sequence-specific translational repressor
Rocaglamide A (RocA) typifies a novel class of protein synthesis inhibitors that selectively kill aneuploid tumor cells and repress translation of specific mRNAs. RocA targets eukaryotic initiation factor 4A (eIF4A), the prototypical DEAD-box RNA helicase, and its mRNA selectivity is proposed to ref...
ORGANISM(S): synthetic construct 
2016-06-15 | E-GEOD-70211 | biostudies-arrayexpress
TranscriptomicsRNA G-quadruplexes cause eIF4A-dependent oncogene translation in cancer
The translational control of oncoprotein expression is implicated in many cancers. Here we report an eIF4A/DDX2 RNA helicase-dependent mechanism of translational control that contributes to oncogenesis and underlies the anticancer effects of Silvestrol and related compounds. For example, eIF4A promo...
ORGANISM(S): Homo sapiens 
2014-07-27 | E-GEOD-56887 | biostudies-arrayexpress
TranscriptomicsRibosome profiling upon inhibition of eIF4A
Ribosome profiling of MDA-MB-231 cells treated with Silvestrol to monitor transcriptome wide, eIF4A-dependent changes in translation efficiency Translation efficiency (TE) of mRNAs dervied from ribosome footprints was monitored in the presence or absence of 25 nM Silvestrol, an inhibitor of eukaryot...
ORGANISM(S): Homo sapiens 
2014-09-13 | E-GEOD-61375 | biostudies-arrayexpress
TranscriptomicsGlucose depletion inhibits translation initiation in yeast - transcription profiling
This is a transcription profiling study on yeast undergoing glucose depletion. It reveals that glucose depletion inhibits translation initiation in a mechanism involving eIF4A loss and 48S pre-initiation complex accumulation, while the pentose phosphate pathway is co-ordinately up-regulated
ORGANISM(S): Saccharomyces cerevisiae 
2011-11-11 | E-MEXP-3037 | biostudies-arrayexpress
TranscriptomicsGlucose depletion inhibits translation initiation in yeast - polysome and monosome profiling
This is a polysome/monosome profiling study on yeast undergoing glucose depletion. Polysomes and monosomes are extracted and the transcripts bound to each fraction are measured by microarray. The study reveals that glucose depletion inhibits translation initiation in a mechanism involving eIF4A loss...
ORGANISM(S): Saccharomyces cerevisiae 
2011-11-11 | E-MEXP-3038 | biostudies-arrayexpress
ProteomicsMapping the Interaction between Eukaryotic Initiation Factor 4A (eIF4A) and the Inhibitor Hippuristanol Using Carbene Footprinting and Mass Spectrometry
Protein-ligand interactions are central to protein activity and cell functionality. Improved knowledge of these relationships greatly benefits our understanding of key biological processes and aids in rational drug design towards the treatment of clinically relevant diseases. Carbene footprinting is...
ORGANISM(S): Homo sapiens (Human) 
2021-08-25 | PXD025105 | Pride
Protein footprinting Mapping ligand binding Carbene labelling 
TranscriptomicsGenome-wide analysis of translational efficiency reveals distinct but overlapping functions of yeast DEAD-box RNA helicases Ded1 and eIF4A
DEAD-box RNA helicases eIF4A and Ded1 are believed to promote translation initiation by resolving mRNA secondary structures that impede ribosome attachment at the mRNA 5’ end or subsequent scanning of the 5’UTR, but whether they perform distinct functions or act redundantly in vivo is poorly underst...
ORGANISM(S): Saccharomyces cerevisiae 
2015-03-02 | E-GEOD-66411 | biostudies-arrayexpress
ProteomicsA SMG7-eIF4A module constitutes a homeostatic system for P-bodies and Meiotic bodies
The nucleation of RNP granules requires the participation of mRNAs, which simultaneously as becoming their target, dictate their properties. Here we report that in Arabidopsis the RNA helicase eIF4A limits the condensation of P-bodies acting as an RNA chaperone, disassembled from the eIF4F complex. ...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2026-03-25 | PXD061406 | Pride
Eif4a Stress granules P-bodies Eif4f Rnp granules Biomolecular condensates Smg7 Rna condensation Meiosis Meiotic bodies 
GenomicsTargeting eIF4A-dependent translation in soft tissue sarcoma
Targeting eIF4A-dependent translation in soft tissue sarcoma
PRJNA946326 | ENA
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