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ProteomicsHST-MRM-MS: A Novel High-Sample-Throughput Multiple Reaction Monitoring Mass Spectrometric Method for Multiplex Absolute Quantitation of Hepatocellular Carcinoma Serum Biomarker
Absolute quantification of clinical biomarkers by mass spectrometry (MS) has been challenged due to low sample-throughput of current multiple reaction monitoring (MRM) methods. For this problem to be overcome, in this work, a novel high-sample-throughput multiple reaction monitoring mass spectrometr...
ORGANISM(S): Homo Sapiens 
2020-02-23 | PXD040708 |
ProteomicsQuantitative analysis of the supernatant from native and transfected CHO cells using iTRAQ 8-plex technique
We employed UPLC-MS/MS with iTRAQ 8-plex labeling to quantitatively analyze the supernatant produced by two Chinese hamster ovary (CHO) cell lines (CHO K1SV and CHO CAT-S). In each case, the supernatant from the host and three transfected clones were analyzed at days 5, 7, and 10 of culture. A total...
ORGANISM(S): Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) 
2016-07-20 | PXD003462 | Pride
Cho supernatant Itraq 8-plex Lc-ms/ms 
UnknownDevelopment and characterization of novel 8-plex DiLeu isobaric labels for quantitative proteomics and peptidomics.
Not available
S-EPMC4837894 | biostudies-literature
UnknownIncreasing the multiplexing capacity of TMTs using reporter ion isotopologues with isobaric masses.
Not available
S-EPMC3715028 | biostudies-literature
ProteomicsSwitchgrass drought-induced leaf proteomics analysis
Switchgrass (Panicum virgatum) is a perennial crop producing deep roots thus highly tolerant to soil water deficit conditions. However, seedling establishment in field is very susceptible to prolonged and periodic drought stress. In this study, a “sandwich” system simulating a gradual water deletion...
ORGANISM(S): Panicum virgatum (Switchgrass) 
2016-08-09 | PXD004675 | Pride
Eight-plex-itraq  proteominer  drought tolerance 
UnknownCapillary isoelectric focusing-tandem mass spectrometry and reversed-phase liquid chromatography-tandem mass spectrometry for quantitative proteomic analysis of differentiating PC12 cells by eight-plex isobaric tags for relative and absolute quantification.
Not available
S-EPMC3759156 | biostudies-literature
UnknownHigh-resolution enabled 12-plex DiLeu isobaric tags for quantitative proteomics.
Not available
S-EPMC4318621 | biostudies-literature
UnknownDifferential proteomics analysis of liver failure in peripheral blood mononuclear cells using isobaric tags for relative and absolute quantitation.
Not available
S-EPMC5351387 | biostudies-literature
UnknownProteomic analysis of grapevine resistance induced by Trichoderma harzianum T39 reveals specific defence pathways activated against downy mildew.
Not available
S-EPMC3481215 | biostudies-literature
ProteomicsMultifactorial Comparative Proteomic Study of Cytochrome P450 2E1 Function in Chronic Alcohol Administration
With the use of iTRAQ technique, a multifactorial comparative proteomic study can be performed. In this study, to obtain an overview of ethanol, CYP2E1 and gender effects on liver injury and gain more insight into the underlying molecular mechanism, mouse liver proteomes were quantitatively analyze...
ORGANISM(S): Mus musculus (Mouse) 
2014-04-11 | PXD000635 | Pride
Alcohol metabolism Liver Cyp2e1 Multifactorial comparative proteome Itraq 
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