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TranscriptomicsSpecificity quantification of Glucocorticoid receptor binding using high-throughput sequencing
In this work, we used our recently developed method Spec-seq to characterize the binding specificity of Glucocorticoid receptor in vitro. This GR Spec-seq experiment has been run twice separately (Dec 2014 and Mar 2015) with different library compositions. The basic workflow is the same as our previ...
ORGANISM(S): Homo sapiens 
2015-07-01 | E-GEOD-69386 | biostudies-arrayexpress
UnknownLabel-Free Electrophoretic Mobility Shift Assay (EMSA) for Measuring Dissociation Constants of Protein-RNA Complexes.
Not available
S-EPMC6391183 | biostudies-literature
UnknownScreening for Functional Non-coding Genetic Variants Using Electrophoretic Mobility Shift Assay (EMSA) and DNA-affinity Precipitation Assay (DAPA).
Not available
S-EPMC5091922 | biostudies-literature
GenomicsThe DNA-binding protein HTa from Thermoplasma acidophilum is an archaeal histone analog (Mnase-seq and EMSA-seq)
The DNA-binding protein HTa from Thermoplasma acidophilum is an archaeal histone analog (Mnase-seq and EMSA-seq)
PRJNA576437 | ENA
TranscriptomicsTargets of the E. histolytica transcription factor URE3-BP
The E. histolytica transcription factor URE3-BP is a calcium-responsive regulator of two E. histolytica virulence genes, hgl5 and fdx1. URE3-BP was previously identified by a yeast one-hybrid screen of E. histolytica proteins capable of binding to the sequence TATTCTATT (URE3) in the promoter region...
ORGANISM(S): Entamoeba histolytica 
2008-10-14 | E-GEOD-12188 | biostudies-arrayexpress
TranscriptomicsThe Bile Response Repressor, BreR, Regulates Expression of the Vibrio cholerae breAB Efflux System Operon
Enteric pathogens have developed several resistance mechanisms to survive the antimicrobial action of bile. We investigated the transcriptional profile of Vibrio cholerae O1 El Tor strain C6706 under virulence gene inducing conditions, in the presence and absence of bile. Microarray analysis reveale...
ORGANISM(S): Vibrio cholerae 
2010-06-05 | E-GEOD-6468 | biostudies-arrayexpress
ProteomicsBLM helicase protein negatively regulates stress granule formation through unwinding RNA G-quadruplex structures
We ran native 5% polyacrylamide-TBE gel for electro mobility shift assay (EMSA) for DNA/RNA G4 structures with or without recombinant human core-BLM (cBLM) protein, which was extracted and purified from E.coli in order to show binding of the RNA/DNA by this protein. To further evidence, we cut the e...
ORGANISM(S): Homo sapiens (Human) 
2023-10-24 | PXD042983 | Pride
Blm Sg Emsa G-quadruplex Lc-ms/ms 
GenomicsSystematic Dissection of Sequence Features Affecting the Binding Specificity of a Pioneer Factor Reveals Binding Synergy Between FOXA1 and AP-1 [EMSA-seq]
Systematic Dissection of Sequence Features Affecting the Binding Specificity of a Pioneer Factor Reveals Binding Synergy Between FOXA1 and AP-1 [EMSA-seq]
PRJNA1037550 | ENA
GenomicsMolecular mechanisms of two-component system RhpRS regulating type III secretion system in Pseudomonas syringae
Pseudomonas syringae uses two-component system RhpRS to regulate the expression of type III secretion system (T3SS) genes and bacterial virulence. However, the molecular mechanism and the regulons of RhpRS have yet to be fully elucidated. Here we show that RhpS functions as an autokinase, an RhpR ki...
ORGANISM(S): Pseudomonas syringae pv. phaseolicola 
2014-10-01 | E-GEOD-58533 | biostudies-arrayexpress
TranscriptomicsGenome-wide EMSA (gEMSA) of ORC and yeast genomic DNA
Eukaryotic DNA replication origins are selected in G1-phase when the origin recognition complex (ORC) binds chromosomal DNA, triggering a series of molecular events that culminate in the initiation of DNA replication (a.k.a. origin firing) during S-phase. Each chromosome requires multiple origins fo...
ORGANISM(S): Saccharomyces cerevisiae 
2013-09-30 | E-GEOD-48440 | biostudies-arrayexpress
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