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MetabolomicsThe Impacts of Sortase A and the 4'-Phosphopantetheinyl Transferase Homolog Sfp on Streptococcus mutans Extracellular Membrane Vesicle Biogenesis

Extracellular membrane vesicles (EMVs) are produced by many Gram-positive organisms, but information regarding vesiculogenesis is incomplete. We used single gene deletions to evaluate the impacts on Streptococcus mutans EMV biogenesis of Sortase A (SrtA), which affects S. mutans...

2021-10-06 | MTBLS1812 | MetaboLights
ProteomicsPlasma and crude membrane fraction from mouse liver and kidney
The purpose of the present study was to validate the enrichment of transporter proteins in plasma membrane fraction prepared from mouse liver and kidney with Membrane Protein Extraction Kit by means of beads homogenize for efficient subcellular fractionation. The crude and plasma membrane fractions ...
ORGANISM(S): Mus Musculus (mouse) 
2020-09-26 | PXD018161 | JPOST Repository
MetabolomicsPlasma metabolomic profiling during peri-parturition in healthy Thoroughbred mares

Background: Accurate prediction of the timing of parturition is crucial to ensure the health and well-being of both mares and foals. However, equine pregnancies are characterized by significant variability in gestation length, unique endocrine mechanisms, and subtle physiological changes before p...

2025-07-07 | MTBLS11326 | MetaboLights
MetabolomicsComparison of the Metabolic Profiles in the Plasma and Urine Samples Between Autistic and Typically Developing Boys: A Preliminary Study
Background: Autism spectrum disorder (ASD) is defined as a pervasive developmental disorder which is caused by genetic and environmental risk factors. Besides the core behavioral symptoms, accumulated results indicate children with ASD also share some metabolic abnormalities. Objectives:
2022-03-02 | MTBLS2421 | MetaboLights
ProteomicsPlasma membrane proteome of adhesion-competent endometrial epithelial cells and its modulation by Rab11a
Human endometrial epithelium (EE) is composed of a multitude of proteins, amongst which those localized on the plasma membrane (plasma membrane proteins-PMPs) are of critical relevance in early stages of implantation. Evidences support the key role of few PMPs in implantation. However, many remain u...
ORGANISM(S): Homo sapiens (Human) 
2019-11-08 | PXD015010 | Pride
Epithelium Plasma membrane Ishikawa Proteomics Endometrium 
TranscriptomicsYeast Membrane Bound Polysome
Set of arrays organized by shared biological context, such as organism, tumors types, processes, etc. mRNA species bound to membrane-associated polysomes were separated from other mRNAs by sedimentation equilibrium or sedimentation velocity. The distribution of individual transcripts in the 'membran...
ORGANISM(S): Saccharomyces cerevisiae 
2005-08-24 | E-GEOD-3180 | biostudies-arrayexpress
ProteomicsArabidopsis seedling FFE-purified plasma membrane LC-MS/MS
Plasma membranes from 7-day old wild type Arabidopsis seedlings were enriched by two-phase partitioning and further purified by free-flow electrophoresis prior to analysis of fractions by LC-MS/MS. Data from two independent replicates were analyzed, with two fractions analyzed from each replicate.
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2015-12-08 | PXD001795 | Pride
Seedling Arabidopsis Free-flow electrophoresis Plasma membrane 
ProteomicsProteomic characterization of plasma membrane-enriched fraction from tobacco (Nicotiana tabacum L. cv Samsun) pollen tubes.
Pollen germination and subsequent pollen tube elongation are essential for successful land plant reproduction. These processes are achieved through well-documented activation of membrane trafficking and cell metabolism. Despite this, our knowledge of the dynamics of cellular phospholipids remains sc...
ORGANISM(S): Nicotiana tabacum (Common tobacco) 
2022-10-27 | PXD037046 | Pride
Pollen tube Tobacco Tip growth Plasma membrane Polarity Pollen 
ProteomicsArabidopsis thaliana root tissue plasma membrane protein enrichment following Endosidin2 treatment
Using a previously identified small molecule inhibitor of the plant exocyst complex subunit EXO70A1, Endosidin2 (ES2), combined with a plasma membrane enrichment method and quantitative proteomic analysis, we examined the composition of plasma membrane proteins in the root of Arabidopsis seedlings, ...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2023-05-18 | PXD037455 | Pride
Arabidopsis thaliana Enriched plasma membrane 
TranscriptomicsImpact of Elevated Plasma 5-HT on Global Gene Expression of Murine Megakaryocytes
The goal of this project was to understand the functional consequences of plasma biogenic amine serotonin (5-HT) elevation in megakaryocytes by measuring gene expression Total RNA was extracted from the megakaryocytes of mice treated with either 5-HT or saline and global gene expression was measured...
ORGANISM(S): Mus musculus 
2014-04-04 | E-GEOD-37273 | biostudies-arrayexpress
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