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ProteomicsERLIC-based strategies for combined quantitative proteome and phosphoproteome analysis with limited sample amounts
The study aims the development of a sensitive strategy for combined quantitative proteomics and phosphoproteomics. 3 ERLIC-based strategies, applicable for low-sample-amount experiments (~150 µg per condition), were evaluated and compared . Three samle sets have been uploaded. 16 fractions ERLIC-1-S...
ORGANISM(S): Homo sapiens (Human) 
2015-02-25 | PXD001241 | Pride
Protein phosphorylation Erlic Quantitative proteomics Multidimensional separation 
ProteomicsHILIC and ERLIC Enrichment of Glycopeptides Derived from Breast and Brain Cancer Cells
Aberrant glycosylation has been linked to many different cancer types. The blood brain barrier (BBB) is a region of the brain that regulates the entrance of ions, diseases, toxins, etc. However in breast cancer metastasis, the BBB fails to prevent the crossing of the cancer cells into the brain. Her...
ORGANISM(S): Homo sapiens (Human) 
2019-02-27 | PXD004361 | Pride
Hilic enrichment Glycoproteomics Erlic enrichment Mass spectrometry Blood brain barrier Cancer cell lines N-linked glycosylation 
ProteomicsFinding the Sweet Spot in ERLIC Mobile Phase for Simultaneous Enrichment of N-Glyco and Phosphopeptides
Simultaneous enrichment of glyco- and phospho- peptides will benefit the studies of biological processes regulated by these post-translational modifications (PTMs). It will also reveal potential crosstalk between these two ubiquitous PTMs. Unlike custom designed multifunctional solid phase extractio...
ORGANISM(S): Homo sapiens (Human) 
2019-09-06 | PXD015179 | Pride
Mm.1s Glycosylation Erlic Phosphorylation Lc-ms/ms 
UnknownAntibody characterization using novel ERLIC-MS/MS-based peptide mapping.
Not available
S-EPMC6204790 | biostudies-literature
ProteomicsAnion-Exchange Chromatography of Tryptic and Phosphopeptides: WAX vs. SAX and AEX vs. ERLIC
At a pH > 5, phosphopeptides have two negative charges per residue and are well-retained in anion-exchange chromatography. However, the peptides with one or two phosphate groups are not separated from the peptides with multiple Asp or Glu residues, which interfere with the identification of phosph...
ORGANISM(S): Homo sapiens (Human) 
2015-04-23 | PXD001333 | Pride
Chromatography erlic sax wax aex 
UnknownOnline nanoscale ERLIC-MS outperforms RPLC-MS for shotgun proteomics in complex mixtures.
Not available
S-EPMC3487405 | biostudies-literature
ProteomicsCounterion Optimization Dramatically Improves Selectivity for Phosphopeptides and Glycopeptides in Electrostatic Repulsion-Hydrophilic Interaction Chromatography (ERLIC)
A well-hydrated counterion can selectively and dramatically increase retention of a charged analyte in hydrophilic interaction chromatography (HILIC). The effect is enhanced if the column is charged, as in electrostatic repulsion-hydrophilic interaction chromatography (ERLIC). This combination was...
ORGANISM(S): Mus musculus (Mouse) 
2021-06-01 | PXD022988 | Pride
Peptide Fraction Ptm Glyco Phosphate Erlic 
ProteomicsPlasma Glycopeptide Enrichment
We have compared three different glycopeptide enrichment techniques (SAX-ERLIC, HILIC, and lectin affinity) and a C18 control to a tryptic digest of proteins from depleted human plasma.
ORGANISM(S): Homo sapiens (Human) 
2017-02-16 | PXD005655 | Pride
Lc-ms Glycopeptide Plasma 
UnknownHILIC and ERLIC Enrichment of Glycopeptides Derived from Breast and Brain Cancer Cells.
Not available
S-EPMC5748158 | biostudies-literature
ProteomicsAnion-Exchange Chromatography of Tryptic and Phosphopeptides: WAX vs. SAX and AEX vs. ERLIC
At a pH > 5, phosphopeptides have two negative charges per residue and are well-retained in anion-exchange chromatography. However, the peptides with one or two phosphate groups are not separated from the peptides with multiple Asp or Glu residues, which interfere with the identification of phosph...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2017-03-29 | MSV000080774 | MassIVE
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