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ProteomicsComparison of the MCF7 proteome cultured in standard FBS and EV depleted FBS
COmparison of the MCF7 proteomes after being cultured for 3 d in either 10% FBS (standard, commercial) or in 10% EV-depleted FBS (EDS, commercial) to validate the absence of EV specific proteins in EDS to exclude potential cross-contamination of xeno-EVs
ORGANISM(S): Bos taurus (Bovine) 
2025-05-06 | PXD051956 | Pride
Ev-depleted fbs Extracellular vesicles Starvation Fbs 
GenomicsQuestioning the purity of the media - Extracellular small non-coding RNA contaminants in fetal bovine serum and serum-free media
In the research field of extracellular vesicles (EVs), the use of EV-depleted fetal bovine serum (FBS) for in vitro studies is highly recommended to eliminate the confounding effects of media derived EVs. EV-depleted FBS may either be prepared by ultracentrifugation or bought commercially, neverthel...
ORGANISM(S): other sequences Bos taurus 
2019-04-30 | GSE120594 | GEO
TranscriptomicsHuman iPSC-derived cardiomyocytes under hypoxia/normoxia, treated with bone marrow or cardiac stromal cell extracellular vesicles
RNA-seq was performed on cultured human induced pluripotent cell derived cardiomyocytes (iPSC-CMs). There are four groups, with three samples per group: H1,H2,H3. Negative control. Healthy, normoxic iPSC-CMs D1,D2,D3. Positive control. iPSCs cultured under hypoxic (0-1% O2) for 48h with 2% v/v PBS ...
ORGANISM(S): Homo sapiens 
2024-10-02 | E-MTAB-13966 | biostudies-arrayexpress
TranscriptomicsSerum extracellular vesicle depletion processes affect release and infectivity of HIV-1 in culture
Extracellular vesicles (EVs) are involved in intercellular communication in health and disease and affect processes including immune and antiviral responses. We have previously demonstrated that ultracentrifuged serum is depleted of EVs and, when used in cell culture media, is associated with declin...
ORGANISM(S): Homo sapiens 
2016-10-23 | GSE89067 | GEO
UnknownEfficient ultrafiltration-based protocol to deplete extracellular vesicles from fetal bovine serum.
Not available
S-EPMC5795649 | biostudies-literature
TranscriptomicsRNA sequencing of extracellular vesicles, secreted by young and adult mouse adipocytes [RNA-Seq]
We used male C57BL/6 mice (Charles River Laboratories, Wilmington, MA), housed under SPF conditions. Primary adipocytes from the inguinal fat depot at postnatal day (P6, young mice) and postnatal day 56 (P56, adult mice) were isolated by collagenase digestion and separation of cell fractions and sub...
ORGANISM(S): Mus musculus 
2024-08-01 | GSE191256 | GEO
GenomicsRNA sequencing of extracellular vesicles, secreted by young and adult mouse adipocytes [miRNA-Seq]
We used male C57BL/6 mice (Charles River Laboratories, Wilmington, MA), housed under SPF conditions. Primary adipocytes from the inguinal fat depot at postnatal day (P6, young mice) and postnatal day 56 (P56, adult mice) were isolated by collagenase digestion and separation of cell fractions and sub...
ORGANISM(S): Mus musculus 
2024-08-01 | GSE193589 | GEO
ProteomicsProteomic profiling of KIT-containing EVs secreted by neoplastic human mast cells (SUB2)
Activating mutations in the receptor KIT promote the dysregulated proliferation of human mast cells (huMCs). The resulting neoplastic huMCs secrete extracellular vesicles (EVs) that can transfer oncogenic KIT among other cargo into recipient cells. Despite potential contributions to diseases, KIT-co...
ORGANISM(S): Homo sapiens (Human) 
2025-12-02 | PXD033890 | Pride
Exosomes Microvesicles Extracellular vesicles Kit Immunocapture Mast cell 
ProteomicsProteomic profiling of KIT-containing EVs secreted by neoplastic human mast cells (SUB1)
Activating mutations in the receptor KIT promote the dysregulated proliferation of human mast cells (huMCs). The resulting neoplastic huMCs secrete extracellular vesicles (EVs) that can transfer oncogenic KIT among other cargo into recipient cells. Despite potential contributions to diseases, KIT-co...
ORGANISM(S): Homo sapiens (Human) 
2023-03-10 | PXD033889 | Pride
Exosomes Microvesicles Extracellular vesicles Kit Immunocapture Mast cell 
UnknownExtracellular small non-coding RNA contaminants in fetal bovine serum and serum-free media.
Not available
S-EPMC6445286 | biostudies-literature
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