Background: Antimicrobial resistance is generally studied using a combination of growth inhibition measurements, sometimes in combination with DNA detection methods. However, the actual proteins that cause resistance such as enzymes, efflux pumps and a lack of porins cannot be detected by these meth...
Background: Antimicrobial resistance is generally studied using a combination of growth inhibition measurements, sometimes in combination with DNA detection methods. However, the actual proteins that cause resistance such as enzymes, efflux pumps and a lack of porins cannot be detected by these meth...
We use Saccharomyces cerevisiae to perform absolute quantitative multi-omics analysis to map interactions of different cellular processes during the yeast cell cycle.
BMDMs of indicated phenotypes were stimulated with LPS for 24h and analyzed or restimulated with LPS for additional 3h and analyzed. In some experiments 1mM itaconate was added at 4h of LPS first stimulation.
We used RNA microarray to investigate the expression of full-time and part-time sheddases and their substrates in basal cell carcinoma when compared to squamous cell carcinoma and normal skin. We aimed to identify the involvement of these sheddases in CD200 ectodomain shedding. RNA was extracted fro...
We used RNA microarray to investigate CD200-mediated apoptosis on the NK92MI celll line. We aimed to identify apoptotic gene signatures. RNA was extracted from NK92MI cells and sent for sequencing. raw sequencing data was then processed in R using 'lumi' and 'limma' packages.
Many bacteria express filaments called type IV pili on their surface, which are involved in motility (twitching), surface adhesion, biofilm formation and DNA uptake (natural transformation). Type IV pili are comprised of a helical assembly of repeating pilin subunits that allow both flexibility and ...
The pregnancy vaginal microbiome contributes to risk of preterm birth, the primary cause of death in children under 5 years of age. Here we describe direct on-swab metabolic profiling by Desorption Electrospray Ionization Mass Spectrometry (DESI-MS) for sample preparation-free characterisation of...
Glaucoma trabecular meshwork (GTM) cells cultured in vitro retain many characteristics of their in situ phenotype. We used isobaric labeling quantitative proteomics to identify proteins that were differentially regulated between glaucomatous and age-matched non-glaucomatous TM (NTM) cells. NTM (n=5)...
