Oculomotor behavior model system is commonly employed to study the neural circuits and the cellular mechanisms underlying neural integration related to motor behavior. The flocculus is a small lobe at the posterior border of the middle cerebellar and involved in motor control to aid in the oculomoto...
Streptomyces flocculus grown on solid R5 media. Extracted after 7 days in 50:50 butanol/ethyl acetate. Dissolved in 50:50 ACN/water and analyzed by UPLC-MS
Two samples were run: (1) purchased streptonigrin from Sigma Aldrich (2) an extract (pH adjusted to 6.5) made from a culture of S. flocculus (known producer of streptonigrin)