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ProteomicsSystem-wide profiling of protein-complexes via size exclusion chromatography– mass spectrometry (SEC-MS)
In living cells most proteins are organized in stable or transient functional assemblies known as protein complexes, which control a multitude of vital cellular processes such as cell cycle progression, metabolism, and signal transduction. System-wide workflows for analysis of protein complexes usin...
ORGANISM(S): Homo sapiens (Human) 
2022-05-06 | PXD018033 | Pride
Protein complex analysis Sec-ms Protein co-elution profiling 
ProteomicsDefining the interactome of CHD4 in fusion-positive rhabdomyosarcoma
The goal of this study was to explore in detail how the chromatin remodeler and NuRD subunit CHD4 controls the oncogenic signature of the tumor driver and fusion protein PAX3-FOXO1 in fusion-positive rhabdomyosarcoma. To this aim, we defined the interactome of CHD4 by LC-MS, identified its location ...
ORGANISM(S): Homo sapiens (Human) 
2020-08-11 | PXD015231 | Pride
Nurd Rh4 Alveolar rhabdomyosarcoma Chd4 
ProteomicsInteractome of the TCF3-HLF fusion protein
Oncogenic chimeric transcription factors are central drivers in cancer. To understand how the TCF3-HLF fusion protein rewires the transcriptional landscape in t(17;19) positive leukemia, functional genetic and proteomic experiments were conducted. In this dataset, the protein-protein interactions of...
ORGANISM(S): Homo sapiens (Human) 
2020-08-05 | PXD014992 | Pride
Flag-tagged fusion protein Ap-ms 
ProteomicsMultiplexed interactome analysis reveals the molecular architecture of the human prefoldin network
The majority of proteins are organized in protein complexes. Protein complexes represent an important cellular organizational layer, which regulate and catalyze most of the cellular processes. Within the field of proteomics several techniques such as Affinity Purification (AP) and co-fractionation (...
ORGANISM(S): Homo sapiens (Human) 
2024-03-11 | PXD035032 | Pride
Co-fractionation based profiling Dia Affinity purification coupled to mass spectrometry Size-based fractionation Protein complexes 
ProteomicsInteraction proteomics of wildtype SLC16A6 (MCT7) and phospho-variants
The solute carrier (SLC) transporter superfamily are important coordinators of metabolic and cellular homeostasis. Despite their importance for cellular homeostasis, SLCs remain a largely understudied group of proteins regarding their protein interactome. Thus, we conducted a largescale interactome ...
ORGANISM(S): Homo sapiens (Human) 
2025-03-03 | PXD055141 | Pride
Phosphodegron Slc16a6 phsopho-variants Ap-ms 
ProteomicsDefining the interactome of ARID5B by AviTag protein pull-down coupled to quantitative mass spectrometry.
Among the strongest associations with pediatric B-cell acute lymphoblastic leukemia (B-ALL) are risk non-coding variants localized in the intron of ARID5B (AT-rich interaction domain 5B), a poorly characterized DNA-binding protein. In this study, we defined the interactome of ARID5B by LC-MS/MS and ...
ORGANISM(S): Homo sapiens (Human) 
2026-05-19 | PXD061867 | Pride
Transcriptional repressor complex Avitag 
ProteomicsSelectivity profiling of SLC16A3/MCT4 inhibitor slCeMM1 using photoaffinity probe
To assess the selectivity of SLC16A3/MCT4 inhibitor slCeMM1, we derived diazirine-alkyne photoaffinity probe (slCeMM1-PAP). We next used HAP1 and MDAMB231 cell lines for MS experiment, comparing the enrichment of proteins by slCeMM1-PAP and conditions where slCeMM1-PAP was competed with slCeMM1 or i...
ORGANISM(S): Homo sapiens (Human) 
2023-10-16 | PXD040089 | Pride
Photoaffinity probe  ap-ms  tmt  chemoproteomics 
ProteomicsWithanolide analogues disrupt a leukemic dependency on cholesterol transport by inhibiting the oxysterol-binding protein OSBP (Expression proteomics)
Metabolic alterations in cancers precipitate in associated dependencies that can be therapeutically exploited. To meet this goal, natural product inspired small molecules can provide a resource of underexplored chemotypes. We identify W7, a synthetic withanolide analog with pronounced anti-leukemic ...
ORGANISM(S): Homo sapiens (Human) 
2024-06-27 | PXD040694 | Pride
Thermal proteome profiling Expression proteomics Withanolides Natural product-inspired compound libraries 
ProteomicsWithanolide analogues disrupt a leukemic dependency on cholesterol transport by inhibiting the oxysterol-binding protein OSBP (TPP)
Metabolic alterations in cancers precipitate in associated dependencies that can be therapeutically exploited. To meet this goal, natural product inspired small molecules can provide a resource of underexplored chemotypes. We identify W7, a synthetic withanolide analog with pronounced anti-leukemic ...
ORGANISM(S): Homo sapiens (Human) 
2024-06-27 | PXD040692 | Pride
Thermal proteome profiling Expression proteomics Withanolides Natural product-inspired compound libraries 
ProteomicsInteraction proteomics of WT and disease variants of the human Creatine Transporter (SLC6A8)
Creatine is an essential metabolite for the storage and rapid supply of energy in muscle and nerve cells. In humans, the creatine deficiency syndrome (CDS) is manifested by impaired metabolism, transport, and distribution of creatine throughout the body leading to severe forms of mental disabilities...
ORGANISM(S): Homo sapiens (Human) 
2024-01-03 | PXD047111 | Pride
Ap-ms Slc6a8 Protein interactions Pathogenic variants 
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