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ProteomicsEmploying Sterculic Acid–a naturally occurring 1, 2-cyclopropene fatty acid–for live-cell imaging of lipids by inverse electron-demand Diels-Alder reaction
To allow the study of unsaturated free fatty acids in live cells, we here report the use of sterculic acid, a 1,2-cyclopropene containing oleic acid analogue, as a bioorthogonal probe. We here show that this lipid can be readily taken up by dendritic cells without toxic side-effects, and that it can...
ORGANISM(S): Mus musculus (Mouse) 
2023-04-28 | PXD033831 | Pride
Lipidation Ptm Bioorthogonal Sterculic Cyclopropene 
ProteomicsProfiling the anandamide reuptake inhibitor WOBE437
Anandamide, or N-arachidonoylethanolamine (AEA), is a signaling lipid that modulates neurotransmitter release via activation of the cannabinoid receptor type 1 (CB1R) in the brain. Termination of anandamide signaling is thought to be mediated via a facilitated cellular reuptake process utilizing an ...
ORGANISM(S): Mus musculus (Mouse) 
2022-05-04 | PXD031488 | Pride
Abpp Photoaffinity Proteomics Endocannabinoid 
ProteomicsEstablishing an activity-based protein profiling platform to screen in situ kinase inhibitor engagement
Activity-based protein profiling is a powerful tool to study target engagement in complex biological environments. XO44 is a chemical probe based on the scaffold of a promiscuous kinase binder, which covalently reacts with a conserved lysine in the ATP-binding pocket of kinases. Pretreatment of cell...
ORGANISM(S): Homo sapiens (Human) 
2025-04-09 | PXD055387 | Pride
Drug discovery Kinase Phosphoproteomics Abpp 
ProteomicsActivity-based protein profiling in human M2 macrophages using DHA-based photoaffinity probes
17-Hydroxydocosahexaenoic acid (17-HDHA), an oxidative metabolite of the fish oil constituent docosahexaenoic acid (DHA, 22:6 n-3), is a signaling lipid with anti-inflammatory properties. The molecular mechanisms underlying the biological effect of 17-HDHA are poorly understood. Here, we report the ...
ORGANISM(S): Homo sapiens (Human) 
2023-04-28 | PXD027600 | Pride
Human Abpp Photoaffinity Macrophage 
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