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ProteomicsA boundary-defining protein facilitates megabase-scale regulatory chromosomal loop formation in Drosophila neurons
Meta-loops are large chromosomal loops that connect specific neuronal genes with non-coding DNA elements, enabling megabase-range regulation of gene transcription in fly neurons. To reveal how highly specific meta-loop interactions form, we developed an unbiased approach to identify proteins from Dr...
ORGANISM(S): Drosophila melanogaster (Fruit fly) 
2025-04-11 | PXD060311 | Pride
Meta-loop Neuron 3d genome organization Cp190 Drosophila Transcription factor Lola 
ProteomicsEssential role of Cp190 in physical and regulatory boundary formation in Drosophila
Animal genomes fold into contact domains defined by enhanced internal contact frequencies with debated functions in establishing independent gene regulatory domains. A large fraction of contact domains in mammals are formed by stalling of chromosomal loop-extruding cohesin by CTCF at domain boundari...
ORGANISM(S): Drosophila melanogaster (Fruit fly) 
2022-05-30 | PXD027795 | Pride
Genome architecture Development Drosophila 
ProteomicsCTCF loss has limited effects on genome architecture in Drosophila despite critical regulatory functions.
Proteins co-purifying with recombinantly purified and N-terminally GFP-3C-tagged CTCF N-terminus (amino acids 1-293) produced in bacteria were identified by incubating the tagged bait with soluble nuclear protein extracts from 0-12h hour-old wildtype (OregonR strain) Drosophila melanogaster embryos.
ORGANISM(S): Drosophila melanogaster (Fruit fly) 
2021-01-18 | PXD019487 | Pride
Genome organization Silencers 
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