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TranscriptomicsSystems Biology Analysis of Tenofovir 1% Gel in a Phase I Rectal Microbicide Trial
In MTN-007, a phase 1, randomized, double-blinded rectal microbicide trial, we used systems genomics/proteomics to determine the effect of tenofovir 1% gel, nonoxynol-9 2% gel, placebo gel or no treatment on rectal biopsies taken at baseline, after one application or after seven daily applications (...
ORGANISM(S): Homo sapiens 
2015-02-23 | E-GEOD-57025 | biostudies-arrayexpress
ProteomicsGel-free/label-free proteomic, biochemical and photosynthetic anaysis of cowpea resistance against Cowpea severe mosaic virus
In this present work, photosynthetic and biochemical parameters in conjunction with gel-free/label-free proteomic analysis were assessed in a resistant cowpea genotype challenged with CPSMV and compared with the corresponding data of the mock-inoculated control as an attempt to find out some clues t...
ORGANISM(S): Vigna unguiculata 
2024-09-12 | PXD005906 | Pride
Cowpea Incompatible interaction Physiology Biochemistry Gel-free/label-free proteomic 
ProteomicsCBB staining showed beneficial effects on proteomic analysis employing PAGE separation followed with whole-gel slicing, in-gel digestion and quantitative LC-MS/MS
Proteomic analysis by combining PAGE separation, gel slicing and slice-by-slice LC-MS/MS has been frequently reported in recent years. Since the MS analysis would provide identities and quantities of the proteins along the whole lane, visualization by dye staining could be skipped to save time and ...
ORGANISM(S): Rattus norvegicus (Rat) 
2019-02-16 | PXD011689 | Pride
Sds-page In-gel digestion Proteomic analysis Cbb r-250 staining Whole gel slicing Quantitative lc-ms/ms Nondenaturing page 
Genomics96 mRRBS libraries: Gel-free multiplexed reduced representation bisulfite sequencing for large-scale DNA
Sequencing based approaches have led to new insights about DNA methylation. While many different techniques for genome-scale mapping of DNA methylation have been employed, throughput has been a key limitation for most. To further facilitate the mapping of DNA methylation, we describe a protocol for ...
ORGANISM(S): Homo sapiens 
2012-08-29 | E-GEOD-40429 | biostudies-arrayexpress
ProteomicsProtein Metabolism Underlying Heat Tolerance in Contrasting Creeping Bentgass Lines: Insights From Gel-Free Proteomics
One of the major disfunctions that occurs in heat-stressed plants is enhanced protein damage and a consequent decline in cellular protein content. Creeping bentgrass (Agrositis stolonifera L.) is an economically important perennial grass species which is largely used on high value turf areas but exp...
ORGANISM(S): Agrostis stolonifera 
2025-10-20 | PXD060809 | Pride
Heat stress  creeping bentgrass  polyubiquitin-omics  gel-free proteomics  proteostasis 
ProteomicsTube-gel digestion: a fast and reproducible sample preparation protocol for label-free quantitative proteomics of complex biological samples
Comparison of tube-gel protocol to stacking gel and liquid digestion for label-free extracted ion-current quantification of complex biological samples Dataset with known fold-changes (UPS1 spiked in yeast background at 25-10-5-2.5-1-0.5 fmol)
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2016-10-18 | PXD003841 | Pride
Label-free quantitative proteomics Liquid digestion Sample preparation Tube-gel Stacking gel 
TranscriptomicsBenign prostate epithelial cell gene expression after four weeks of treatment with daily transdermal DHT or placebo gel
Significant increases in serum DHT do not increase intraprostatic levels of DHT, PSA, prostate volume, or prostate epithelial cell androgen-regulated gene expression in healthy men. Changes in serum androgen concentrations are not necessarily mimicked within the prostate. Custom Agilent 44K whole hu...
ORGANISM(S): Homo sapiens 
2011-01-19 | E-GEOD-20317 | biostudies-arrayexpress
ProteomicsGel based and gel-free search for plasma membrane proteins in chickpea (Cicer arietinum L.) augments the comprehensive data sets of membrane protein repertoire
Plasma membrane (PM) encompasses total cellular contents, serves as a living barrier regulates all cellular exchanges in a spatiotemporal fashion. Most of the essential tasks of PMs including molecular transport, cell-cell interaction and signal transduction are carried out by their proteinaceous co...
ORGANISM(S): Cicer arietinum (Chickpea) (Garbanzo) 
2024-12-05 | PXD003697 | Pride
Shotgun proteomics Plant proteomics High resolution mass spectrometry 
GenomicsGel-free library preparation for next-generation RNA sequencing and small RNA quantification
Gel-free library preparation for next-generation RNA sequencing and small RNA quantification
PRJNA1379658 | ENA
ProteomicsAn alliance of gel-based and gel-free proteomic techniques displays substantial insight into the proteome of a virulent and an attenuated Histomonas meleagridis strain
The current work aimed to detect and identify significant differentially expressed proteins between a virulent and an attenuated Histomonas meleagridis strain which was in vitro co-cultivated with Escherichia coli DH5alpha. This was done by applying 2D-DIGE and sequential window acquisition of all t...
ORGANISM(S): Histomonas meleagridis 
2018-11-22 | PXD009332 | Pride
Attenuation. Protozoa Swath ms Histomonas meleagridis Virulence factors Comparative proteomic analysis 2d-dige Maldi-tof/tof Lc-ms/ms 
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