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ProteomicsGephyrin interactome from mouse brain lysates using anti-gephyrin antibody and anti-gephyrin DARPins
In order to understand the protein interactors of the inhibitory synaptic scaffolding protein gephyrin, we precipitated gephyrin protein complexes from adult mouse brain lysates of both sexes using both a commercial anti-gephyrin antibody (clone 3B11), as well as anti-gephyrin Designed Ankyrin Repea...
ORGANISM(S): Mus musculus (Mouse) 
2022-11-01 | PXD033641 | Pride
Ap-ms Mus musculus 3b11 C56bl/6j mouse Gephyrin Gphn Designed ankyrin repeat domain Darpin Interactome Lc-ms/ms 
ProteomicsIdentifying altered mouse gephyrin interactome after sleep deprivation
GABAergic transmission controls cortical sleep, however the molecular determinants of GABAergic control of this phenomenon are unknown. We used immunoprecipitation of the inhibitory postsynaptic protein gephyrin to identify gephyrin interactors at ZT6 (sleep) and ZT6 with sleep deprivation (ZT6SD).
ORGANISM(S): Mus musculus (Mouse) 
2024-07-25 | PXD048929 | Pride
Mouse Gephyrin interactome Sleep deprivation 
UnknownA DARPin-based molecular toolset to probe gephyrin and inhibitory synapse biology.
Not available
S-EPMC9674349 | biostudies-literature
UnknownDiazepam Accelerates GABAAR Synaptic Exchange and Alters Intracellular Trafficking.
Not available
S-EPMC6497791 | biostudies-literature
ProteomicsA gephyrin-like protein links cell division and polar elongation in Corynebacteriales
To discover missing players in corynebacterial cell division we used mass-spectrometry based interactomics using formaldehyde as crosslinker, starting with the FtsZ membrane anchor SepF as a bait for co-immunoprecipitation (co-IP) of divisome members. For SepF interactome we carried out the co-IPs u...
ORGANISM(S): Corynebacterium glutamicum ATCC 13032 
2023-07-26 | PXD037255 | Pride
Cell division Corynebacterium glutamicum Interactomics 
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