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ProteomicsMALDI-MS Profiling of Intact Blood Proteins without the Need for Freezing - Dried Serum Spots as Future Clinical Tools for Patient Screening
To open new ways for MALDI-MS-based patient screening, blood serum is the most preferred specimen because of its richness in patho-physiological information and due to ease of collection. To overcome deleterious freeze / thaw cycles and to reduce high costs for shipping and storage, we sought to dev...
ORGANISM(S): Homo sapiens (Human) 
2021-05-20 | PXD024669 | Pride
Intact serum protein analysis Maldi-ms profiling Fast frozen serum Serum proteolysis analysis Dried serum spots 
ProteomicsIntact Transition Epitope Mapping – Serological Inspection by Epitope EXtraction (ITEM – SIX)
Precision medicine requests accurate serological inspections to precisely stratify patients for targeted treatment. Intact transition epitope mapping analysis proved seroconversion of a model organism's serum when spiked with a monoclonal murine anti-ovalbumin antibody with epitope-resolution. Isola...
ORGANISM(S): Gallus gallus (Chicken) 
2023-03-31 | PXD040754 | Pride
Epitope mapping Item-six Blood serum Seroconversion Offline nanoesi-ms Immune complex analysis 
ProteomicsIntact Transition Epitope Mapping - Forces of Interactions between Variants of Equivalent macromolecules (ITEM-FIVE)
Foldon (F) is a specific partial sequence from the C-terminal domain of T4 fibritin, which is found at the neck of the T4 bacteriophage. The amino acid sequence (27 residues) in single letter code is GYIPEAPRDGQAYVRKDGEWVLLSTFL. In neutral solution (pH 6.9) it forms a homo-trimer (F-F-F). Biotinylat...
ORGANISM(S): Enterobacteria phage T4 (Bacteriophage T4) 
2024-04-29 | PXD044721 | Pride
Item-two Hetero-trimer Offline esi-ms Foldon Complex dissociation Homo-trimer Gas phase 
ProteomicsIntact Transition Epitope Mapping- Thermodynamic Weak-force Order (ITEM-TWO)
Most important characteristics of antibodies are that they typically strongly bind to specific epitopes, thereby expressing low dissociation constants (KDs) and high Gibbs free binding energies (delta Gs). In this study, we describe an off-line nano-electrospray mass spectrometry method, termed IT...
ORGANISM(S): Homo sapiens (Human) 
2019-11-07 | PXD016024 | Pride
Antibody affinity determination Antibody specificity determination 
ProteomicsIntact Transition Epitope Mapping - Force differences between Original and Unusual Residues (ITEM-FOUR)
Human cardiac Troponin I epitope peptides were incubated with monoclonal anti-cardiac Troponin I antibody. Immune complexes were subjected to nano electrospray mass spectrometry in order to determine apparent binding energies and dissociation constants of immune complex dissociation reactions in the...
ORGANISM(S): Homo sapiens (Human) 
2023-01-17 | PXD038365 | Pride
Item-four Troponin i Offline nanoesi-ms 
ProteomicsMemory-enhancing effects of Origanum majorana essential oil in an Alzheimer's amyloid beta1-42 rat model: a molecular and behavioral study
Origanum L. (Lamiaceae) is an important genus of medicinal and aromatic plants used in the traditional medicine since ancient times as culinary herbs and remedies. The effects of Origanum majorana-based aromatherapy was investigated in an Alzheimer's amyloid beta1-42 rat model. Protein extracts from...
ORGANISM(S): Rattus norvegicus (Rat) 
2020-09-29 | PXD021082 | Pride
Alzheimer disease Rat Amyloid beta1-42 Brain tissue Origanum majorana 
ProteomicsMass spectrometric ITEM-ONE analysis confirms and refines an assembled affimer binding site on the HER-2 targeting therapeutic antibody Pertuzumab
The Pertuzumab-binding affimer 00557_709097 was incubated either with peptides from tryptically digested Pertuzumab or with selected synthesized peptides. Affimer-peptide complexes were subjected to nano electrospray mass spectrometric ITEM analyses in order to identify the affimer’s epitope on Pert...
ORGANISM(S): Homo sapiens (Human) 
2024-04-09 | PXD043203 | Pride
Pertuzumab Item Affimer Offline nanoesi-ms 
ProteomicsMass Spectrometric Analysis of Antibody-Epitope Peptide Complex Dissociation: Theoretical Concept and Practical Procedure of Binding Strength Characterization
We describe theoretical concepts and explain practical procedures of an electrospray mass spectrometry method, termed “Intact Transmission Epitope Mapping – Thermodynamic Weak-force Order (ITEM-TWO)”, by which can be determined apparent binding energies and dissociation constants of immune complex ...
ORGANISM(S): Homo sapiens (Human) Equus caballus (Horse) 
2020-10-29 | PXD021296 | Pride
Protocol Item-two Rnase s Offline esi-ms Troponin i epitope peptide-antitroponin i antibody complex Myoglobin Flag-peptide–antiflag antibody complex 
ProteomicsIntact Transition Epitope Mapping-Targeted High-Energy Rupture of Extracted Epitopes (ITEM-THREE)
ITEM-THREE is a novel mass spectrometry-based epitope mapping method. To perform ITEM-THREE, antibody-epitope peptide immune-complexes are first generated in electrospray-compatible solutions in which the antibodies maintain their in-solution functionality, by adding an antibody of interest to a mi...
ORGANISM(S): Homo sapiens (Human) 
2019-06-03 | PXD013823 | Pride
Human Molecular recognition Nanoesi Recombinant proteins Ion mobility separation Item-three Collision induced dissociation Epitope antibody complexes 
ProteomicsCharacterization of the Zein Protein Composition in Maize Flour Extracts
Highly homogenous zein protein was isolated from maize kernels in an environment-friendly process using 95 % ethanol as solvent. High purity of the zein protein product was determined by SDS PAGE analysis and by 2 D gel electrophoresis followed by MALDI-ToF-MS peptide mass fingerprinting after in-ge...
ORGANISM(S): Zea mays (Maize) 
2019-06-11 | PXD012849 | Pride
Zein proteins Peptide mass fingerprinting Data base search Maize Maldi-ms Custom-built sequence data base Flour 2d gel electrophoresis 
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