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ProteomicsIdentification of human PKD2 interaction partners from Golgi-enriched protein fractions of HeLa cells by affinity enrichment cross-linking/MS
We investigated the interaction network of human PKD2 in the cytosol as well as in Golgi-enriched subcellular protein fractions, using an affinity enrichment strategy combined with chemical cross-linking/mass spectrometry (MS). Analysis of the subproteomes revealed the presence of distinct proteins ...
ORGANISM(S): Homo sapiens (Human) 
2016-09-27 | PXD003913 | Pride
Human Pkd2 Hela cells Cross-linking/ms Affinity enrichment Golgi apparatus Orbitrap fusion 
MetabolomicsSeipin Governs Caveolin-1 Trafficking Through Modulating Sphingolipid-Glycerolipid Balance

Dysregulation of Caveolin-1 (CAV1), a key component of caveolae, leads to pleiotropic disorders; yet, the mechanisms controlling its trafficking remain unclear. Here, we show that the lipid droplet (LD) biogenesis factor seipin controls CAV1 localization. Seipin deficiency in mice and HeLa cells ...

2025-07-22 | MTBLS12759 | MetaboLights
ProteomicsLabel-free proteomics of immuno-purified SYP61-trans-Golgi Network compartment in control condition and upon sphingolipid acyl-chain length reduction by metazachlor.
The trans-Golgi Network (TGN) in plants is a main sorting hub for proteins and lipids. The lipid composition at the TGN is instrumental to the polar sorting of the auxin efflux carrier PIN2 from TGN to the apical membrane. Here, we immuno-purified the full and intact TGN using the TGN-resident synta...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2021-12-01 | PXD026252 | Pride
Arabidopsis thaliana Sphingolipid Acyl-chain length Free proteomics Immuno-purification Metazachlor Trans-golgi network 
ProteomicsImpact of mannosylation on structure and functionality of ER and Golgi
Many proteins undergo glycosylation in the endoplasmic reticulum (ER) and the Golgi apparatus. Altered glycosylation can manifest in serious, sometimes fatal malfunctions. We recently showed that mutations in the cytoplasmic protein GDP-mannose pyrophosphorylase A (GMPPA) cause a syndrome characteri...
ORGANISM(S): Mus musculus (Mouse) 
2023-03-11 | PXD037129 | Pride
Golgi network  endoplasmic reticulum  mannosylation 
ProteomicsSyntaxin5’s flexibility in SNARE pairing supports Golgi functions
The intracellular transport system is an evolutionally conserved, essential, and highly regulated network of organelles and small transport carriers that traffic protein and lipid cargoes within the cell. The Conserved Oligomeric Golgi (COG) complex is the major Golgi Multisubunit Tethering Complex....
ORGANISM(S): Homo sapiens (Human) 
2023-06-26 | PXD036741 | Pride
Membrane trafficking Golgi Bet1l Vti1b Snap29 Gor1 Gosr2 Cog Snares Stx5 Ykt6 
GenomicsAging-associated increase in Golgi stress disturbs cellular signaling and epigenetic regulation
Aging-associated increase in Golgi stress disturbs cellular signaling and epigenetic regulation
PRJNA986093 | ENA
ProteomicsIdentification of human PKD2 interaction partners from cytosolic protein fractions of HeLa cells by affinity enrichment cross-linking/MS
We investigated the interaction network of human PKD2 in the cytosol as well as in Golgi-enriched subcellular protein fractions, using an affinity enrichment strategy combined with chemical cross-linking/mass spectrometry (MS). Analysis of the subproteomes revealed the presence of distinct proteins ...
ORGANISM(S): Homo sapiens (Human) 
2016-09-27 | PXD003909 | Pride
Human Pkd2 Cross-linking/ms Affinity enrichment Orbitrap fusion 
ProteomicsBioID performed on Golgi-enriched fractions identify C10orf76 as a GBF1 Binding Protein essential for Golgi maintenance and secretion
Data contains LC-MS data of proximity-based biotin labeling assay (BioID) to generate the protein interaction network of GBF1 protein fused to FlagBirA (N-terminal tag) and expressed in HeLa cells and extracted from whole cell lysates and Golgi fractions.
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2019-05-27 | MSV000083866 | MassIVE
UnknownGolgi-associated retrograde protein (GARP) complex-dependent endosomes to trans Golgi network retrograde trafficking is controlled by Rab4b.
Not available
S-EPMC11020649 | biostudies-literature
UnknownGolgi-localized LOT regulates trans-Golgi network biogenesis and pollen tube growth.
Not available
S-EPMC6275481 | biostudies-literature
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