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ProteomicsAnalysis of endogenous immuno-purification of YAP1 in HEK293A cell lines
Analysis of endogenous immuno-purification of YAP1 in HEK293A cell lines
ORGANISM(S): Homo sapiens (Human) 
2023-03-04 | PXD030137 | Pride
Endogenous apms 
ProteomicsUrea artifacts interfere with immunopurification of lysine acetylation
Urea-based buffers can induce carbamylation artefacts. Here we report that carbamylation of lysine residues resembles the structure of acetylated lysines (K-ac) and compete during K-ac immunoaffinity purification. We propose an alternative strategy using an ionic detergent which improves significant...
ORGANISM(S): Homo sapiens (Human) 
2017-01-11 | PXD003701 | Pride
Urea Immunopurification Acetyl Acetylation Carbamylation Carbamyl 
ProteomicsProteasome resides in and dismantles plant heat stress granules constitutively
Stress granules (SGs) are conserved reversible cytoplasmic condensates enriched with aggregation-prone proteins assembled in response to various stresses. Defects in disassembly have been associated with various neuro- and muscular degenerative diseases in humans. Plants appear to have efficient app...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2024-08-22 | PXD044922 | Pride
Stress granules (sg) Proteasomes Heat stress Sg disassembly 
TranscriptomicsLong-term effect on the transcriptome of loss of Frizzled4 signaling in cerebellar endothelial cells
Transcriptional profiles of the cerebellar endothelial cells from P16 Fz4-/- animals were compared to their wild type littermate controls. The goal is to characterize the long-term effect on the transcriptome of loss of Fz4 signaling in cerebellar endothelial cells. Endothelial cells were immuno-pur...
ORGANISM(S): Mus musculus 
2009-10-01 | E-GEOD-16707 | biostudies-arrayexpress
UnknownTandem immuno-purification of affinity-tagged proteins from mouse testis extracts for MS analysis.
Not available
S-EPMC9201064 | biostudies-literature
ProteomicsA cross-linking-aided PRM mass spectrometry for EGFR proteome
Quantification of EGFR complex component from Formaldehyde cross-linked immuno-purification using PRM method
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2018-08-08 | MSV000082800 | MassIVE
UnknownPurification and identification of trichloroethylene induced proteins from Stenotrophomonas maltophilia PM102 by immuno-affinity-chromatography and MALDI-TOF Mass spectrometry.
Not available
S-EPMC3664754 | biostudies-literature
ProteomicsDi-glycine immunopurification proteomics of human skin
We performed profiling of Ubiquitin modifications in human skin which we compared to clinically relevant patient samples that model dysregulated ubiquitination, CYLD cutaneous syndrome (CCS) tumours. We utilised protein peptide di-glycine immuno-purification to enrich for ubiquitinated substrates, f...
ORGANISM(S): Homo sapiens (Human) 
2026-09-18 | PXD069159 | Pride
UnknownPurification of soluble HLA class I complexes from human serum or plasma deliver high quality immuno peptidomes required for biomarker discovery.
Not available
S-EPMC5557337 | biostudies-literature
UnknownPurification, Cloning and Immuno-Biochemical Characterization of a Fungal Aspartic Protease Allergen Rhi o 1 from the Airborne Mold Rhizopus oryzae.
Not available
S-EPMC4682942 | biostudies-literature
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