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ProteomicsCharacterization of the proteome of human blood platelet sercretory granules using gas-phase fractionation (GPF)
We performed an extensive qualitative characterization of the platelet granule proteome using subcellular fractionation followed by mass spectrometry analysis and functional annotation. Platelets were isolated from whole blood by centrifugation and their level of purity was tested by microscopic ins...
ORGANISM(S): Homo sapiens (Human) 
2014-03-06 | PXD000618 | Pride
Platelet Secretory granules Gpf4 
ProteomicsImproving the proteome coverage of Daphnia magna
To improve proteome coverage in Daphnia, we established an easy-to-use procedure based on the LC-MS/MS of whole daphnids and dissected daphnia guts separately. Using a comprehensive spectral library, generated by gas-phase fractionation (GPF) and a data-independent acquisition method, we identified ...
ORGANISM(S): Daphnia magna Sus scrofa domesticus (domestic pig) 
2022-11-17 | PXD029198 | Pride
Dia Mass spectrometry Gut Aquatic pollution Ecotoxicoproteomics Daphnia 
ProteomicsExamining pathways of iron and sulfur acquisition, trafficking, deployment, and storage in mineral-grown methanogen cells
Methanogens were recently shown to reduce pyrite (FeS2) generating aqueous iron-sulfide (FeS(aq)) clusters that are likely assimilated as a source of Fe and S. Here, we compare the phenotype of Methanococcus voltae when grown with FeS2 or ferrous iron (Fe(II)) and sulfide (HS-). Differential proteom...
ORGANISM(S): Methanococcus voltae A3 
2021-07-07 | PXD024933 | Pride
Iron-sulfur cluster Pyrite Methanogen Fes2 Fes Mackinawite 
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